Proteomics of secretory-stage and maturation-stage enamel of genetically distinct mice

Senda Charone, Aline De Lima Leite, Camila Peres-Buzalaf, Mileni Silva Fernandes, Lucas Ferreira De Almeida, Marcia Sirlene Zardin Graeff, Rodrigo Cardoso De Oliveira, Ana Paula Campanelli, Sonia Groisman, Gary Milton Whitford, Eric T. Everett, Marília Afonso Rabelo Buzalaf

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The mechanisms by which excessive ingestion of fluoride (F) during amelogenesis leads to dental fluorosis (DF) are still not precisely known. Inbred strains of mice vary in their susceptibility to develop DF, and therefore permit the investigation of underlying molecular events influencing DF severity. We employed a proteomic approach to characterize and evaluate changes in protein expression from secretory-stage and maturation-stage enamel in 2 strains of mice with different susceptibilities to DF (A/J, i.e. 'susceptible' and 129P3/J, i.e. 'resistant'). Weanling male and female susceptible and resistant mice fed a low-F diet were divided into 2 F-water treatment groups. They received water containing 0 (control) or 50 mg F/l for 6 weeks. Plasma and incisor enamel was analyzed for F content. For proteomic analysis, the enamel proteins extracted for each group were separated by 2-dimensional electrophoresis and subsequently characterized by liquid-chromatography electrospray-ionization quadrupole time-of-flight mass spectrometry. F data were analyzed by 2-way ANOVA and Bonferroni's test (p < 0.05). Resistant mice had significantly higher plasma and enamel F concentrations when compared with susceptible mice in the F-treated groups. The proteomic results for mice treated with 0 mg F/l revealed that during the secretory stage, resistant mice had a higher abundance of proteins than their susceptible counterparts, but this was reversed during the maturation stage. Treatment with F greatly increased the number of protein spots detected in both stages. Many proteins not previously described in enamel (e.g. type 1 collagen) as well as some uncharacterized proteins were identified. Our findings reveal new insights regarding amelogenesis and how genetic background and F affect this process.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalCaries Research
Volume50
Issue number1
DOIs
StatePublished - Mar 1 2016

Fingerprint

Dental Enamel
Dental Fluorosis
Proteomics
Amelogenesis
Proteins
Inbred Strains Mice
Water Purification
Incisor
Collagen Type I
Fluorides
Liquid Chromatography
Electrophoresis
Mass Spectrometry
Analysis of Variance
Eating
Diet
Water

Keywords

  • Dental fluorosis
  • Enamel
  • Fluoride
  • Genetic susceptibility
  • Proteomics

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Charone, S., De Lima Leite, A., Peres-Buzalaf, C., Silva Fernandes, M., Ferreira De Almeida, L., Zardin Graeff, M. S., ... Buzalaf, M. A. R. (2016). Proteomics of secretory-stage and maturation-stage enamel of genetically distinct mice. Caries Research, 50(1), 24-31. https://doi.org/10.1159/000442301

Proteomics of secretory-stage and maturation-stage enamel of genetically distinct mice. / Charone, Senda; De Lima Leite, Aline; Peres-Buzalaf, Camila; Silva Fernandes, Mileni; Ferreira De Almeida, Lucas; Zardin Graeff, Marcia Sirlene; Cardoso De Oliveira, Rodrigo; Campanelli, Ana Paula; Groisman, Sonia; Whitford, Gary Milton; Everett, Eric T.; Buzalaf, Marília Afonso Rabelo.

In: Caries Research, Vol. 50, No. 1, 01.03.2016, p. 24-31.

Research output: Contribution to journalArticle

Charone, S, De Lima Leite, A, Peres-Buzalaf, C, Silva Fernandes, M, Ferreira De Almeida, L, Zardin Graeff, MS, Cardoso De Oliveira, R, Campanelli, AP, Groisman, S, Whitford, GM, Everett, ET & Buzalaf, MAR 2016, 'Proteomics of secretory-stage and maturation-stage enamel of genetically distinct mice', Caries Research, vol. 50, no. 1, pp. 24-31. https://doi.org/10.1159/000442301
Charone S, De Lima Leite A, Peres-Buzalaf C, Silva Fernandes M, Ferreira De Almeida L, Zardin Graeff MS et al. Proteomics of secretory-stage and maturation-stage enamel of genetically distinct mice. Caries Research. 2016 Mar 1;50(1):24-31. https://doi.org/10.1159/000442301
Charone, Senda ; De Lima Leite, Aline ; Peres-Buzalaf, Camila ; Silva Fernandes, Mileni ; Ferreira De Almeida, Lucas ; Zardin Graeff, Marcia Sirlene ; Cardoso De Oliveira, Rodrigo ; Campanelli, Ana Paula ; Groisman, Sonia ; Whitford, Gary Milton ; Everett, Eric T. ; Buzalaf, Marília Afonso Rabelo. / Proteomics of secretory-stage and maturation-stage enamel of genetically distinct mice. In: Caries Research. 2016 ; Vol. 50, No. 1. pp. 24-31.
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