Pulmonary alveolar proteinosis. Staining for surfactant apoprotein in alveolar proteinosis and in conditions simulating it

Gurmukh Singh, S. L. Katyal, C. W.M. Bedrossian, R. M. Rogers

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Formalin fixed paraffin-embedded lung tissues (n = 13) or lavage material (n = 4) from 17 patients in whom alveolar proteinosis was the primary disease and ten patients with other primary diagnoses but lung morphology similar to alveolar proteinosis were examined. The tissues were stained by the periodic acid-Schiff method and by the immunoperoxidase method for surfactant specific apoprotein. The intra-alveolar material in patients with primary alveolar proteinosis stained uniformly for surfactant specific apoprotein, whereas the staining was focal in patients with other primary diseases associated with intra-alveolar accumulation of proteinacious material. In both situations, the number of inflammatory cells, especially macrophages, was small. These observations extend an earlier impression about the presence of surfactant specific apoprotein in alveolar spaces in patients with primary alveolar proteinosis and provide a distinction between primary alveolar proteinosis and morphologically similar appearance due to other causes, ie, secondary alveolar proteinosis. The lack of macrophages in the affected tissue appears to be the major pathogenetic factor in alveolar proteinosis.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalChest
Volume83
Issue number1
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

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Pulmonary Alveolar Proteinosis
Apoproteins
Surface-Active Agents
Staining and Labeling
Macrophages
Lung
Periodic Acid
Therapeutic Irrigation
Paraffin
Formaldehyde
Cell Count

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Pulmonary alveolar proteinosis. Staining for surfactant apoprotein in alveolar proteinosis and in conditions simulating it. / Singh, Gurmukh; Katyal, S. L.; Bedrossian, C. W.M.; Rogers, R. M.

In: Chest, Vol. 83, No. 1, 01.01.1983, p. 82-86.

Research output: Contribution to journalArticle

Singh, Gurmukh ; Katyal, S. L. ; Bedrossian, C. W.M. ; Rogers, R. M. / Pulmonary alveolar proteinosis. Staining for surfactant apoprotein in alveolar proteinosis and in conditions simulating it. In: Chest. 1983 ; Vol. 83, No. 1. pp. 82-86.
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