Abstract
Vascular cell hyperproliferation and metabolic reprogramming contribute to the pathophysiology of pulmonary arterial hypertension (PAH). An important cause of PAH in children with congenital heart disease (CHD) is increased pulmonary blood flow (PBF). To better characterize this disease course we studied early changes in pulmonary artery smooth muscle cell (PASMC) proliferation and metabolism using a unique ovine model of pulmonary overcirculation. Consistent with PAH in adults, PASMCs derived from 4-wk-old lambs exposed to increased PBF (shunt) exhibited increased rates of proliferation. While shunt PASMCs also exhibited significant decreases in mitochondrial oxygen consumption, membrane potential, and tricarboxylic acid (TCA) cycle function, suggesting a switch to Warburg metabolism as observed in advanced PAH in adults, they unexpectedly demonstrated decreased glycolytic lactate production, likely due to enhanced flux through the pentose phosphate pathway (PPP). This may be a response to the marked increase in NADPH oxidase (Nox) activity and decreased NADPH/NADP+ ratios observed in shunt PASMCs. Consistent with these findings, pharmacological inhibition of Nox activity preferentially slowed the growth of shunt PASMCs in vitro. Our results therefore indicate that PASMC hyperproliferation is observed early in the setting of pulmonary overcirculation and is accompanied by a unique metabolic profile that is independent of HIF-1α, PDHK1, or increased glycolytic flux. Our results also suggest that Nox inhibition may help prevent pulmonary overcirculation-induced PAH in children born with CHD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | H944-H957 |
| Journal | American Journal of Physiology - Heart and Circulatory Physiology |
| Volume | 311 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 2016 |
Fingerprint
Keywords
- Glycolysis
- Mitochondria
- Oxygen consumption
- Pulmonary overcirculation
- ROS
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)
Cite this
Pulmonary artery smooth muscle cell hyperproliferation and metabolic shift triggered by pulmonary overcirculation. / Boehme, Jason; Sun, Xutong; Tormos, Kathryn V.; Gong, Wenhui; Kellner, Manuela; Datar, Sanjeev A.; Kameny, Rebecca Johnson; Yuan, Jason X.J.; Raff, Gary W.; Fineman, Jeffrey R.; Black, Stephen Matthew; Maltepe, Emin.
In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 311, No. 4, 01.01.2016, p. H944-H957.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pulmonary artery smooth muscle cell hyperproliferation and metabolic shift triggered by pulmonary overcirculation
AU - Boehme, Jason
AU - Sun, Xutong
AU - Tormos, Kathryn V.
AU - Gong, Wenhui
AU - Kellner, Manuela
AU - Datar, Sanjeev A.
AU - Kameny, Rebecca Johnson
AU - Yuan, Jason X.J.
AU - Raff, Gary W.
AU - Fineman, Jeffrey R.
AU - Black, Stephen Matthew
AU - Maltepe, Emin
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Vascular cell hyperproliferation and metabolic reprogramming contribute to the pathophysiology of pulmonary arterial hypertension (PAH). An important cause of PAH in children with congenital heart disease (CHD) is increased pulmonary blood flow (PBF). To better characterize this disease course we studied early changes in pulmonary artery smooth muscle cell (PASMC) proliferation and metabolism using a unique ovine model of pulmonary overcirculation. Consistent with PAH in adults, PASMCs derived from 4-wk-old lambs exposed to increased PBF (shunt) exhibited increased rates of proliferation. While shunt PASMCs also exhibited significant decreases in mitochondrial oxygen consumption, membrane potential, and tricarboxylic acid (TCA) cycle function, suggesting a switch to Warburg metabolism as observed in advanced PAH in adults, they unexpectedly demonstrated decreased glycolytic lactate production, likely due to enhanced flux through the pentose phosphate pathway (PPP). This may be a response to the marked increase in NADPH oxidase (Nox) activity and decreased NADPH/NADP+ ratios observed in shunt PASMCs. Consistent with these findings, pharmacological inhibition of Nox activity preferentially slowed the growth of shunt PASMCs in vitro. Our results therefore indicate that PASMC hyperproliferation is observed early in the setting of pulmonary overcirculation and is accompanied by a unique metabolic profile that is independent of HIF-1α, PDHK1, or increased glycolytic flux. Our results also suggest that Nox inhibition may help prevent pulmonary overcirculation-induced PAH in children born with CHD.
AB - Vascular cell hyperproliferation and metabolic reprogramming contribute to the pathophysiology of pulmonary arterial hypertension (PAH). An important cause of PAH in children with congenital heart disease (CHD) is increased pulmonary blood flow (PBF). To better characterize this disease course we studied early changes in pulmonary artery smooth muscle cell (PASMC) proliferation and metabolism using a unique ovine model of pulmonary overcirculation. Consistent with PAH in adults, PASMCs derived from 4-wk-old lambs exposed to increased PBF (shunt) exhibited increased rates of proliferation. While shunt PASMCs also exhibited significant decreases in mitochondrial oxygen consumption, membrane potential, and tricarboxylic acid (TCA) cycle function, suggesting a switch to Warburg metabolism as observed in advanced PAH in adults, they unexpectedly demonstrated decreased glycolytic lactate production, likely due to enhanced flux through the pentose phosphate pathway (PPP). This may be a response to the marked increase in NADPH oxidase (Nox) activity and decreased NADPH/NADP+ ratios observed in shunt PASMCs. Consistent with these findings, pharmacological inhibition of Nox activity preferentially slowed the growth of shunt PASMCs in vitro. Our results therefore indicate that PASMC hyperproliferation is observed early in the setting of pulmonary overcirculation and is accompanied by a unique metabolic profile that is independent of HIF-1α, PDHK1, or increased glycolytic flux. Our results also suggest that Nox inhibition may help prevent pulmonary overcirculation-induced PAH in children born with CHD.
KW - Glycolysis
KW - Mitochondria
KW - Oxygen consumption
KW - Pulmonary overcirculation
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=84990053190&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990053190&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.00040.2016
DO - 10.1152/ajpheart.00040.2016
M3 - Article
C2 - 27591215
AN - SCOPUS:84990053190
VL - 311
SP - H944-H957
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 4
ER -