Pulmonary lysozyme - A secretory protein of type II pneumocytes in the rat

Gurmukh Singh, S. L. Katyal, W. E. Brown, D. L. Collins, R. J. Mason

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A 14 kDa basic protein isolated from rat lung lavage was demonstrated to be lysozyme by its amino acid sequence analysis. An antiserum to rat lysozyme stained type II pneumocytes and alveolar macrophages. In rat lungs, no staining of the airway cells was noted. Lysozyme was detectable in type II pneumocytes by immunocytochemistry and by a quantitative immunoassay of lung homogenates of fetal lungs at Day 20 of gestation. An increase in the lysozyme content of the lung with increasing gestational and postnatal age of the rat was noted. In adult animals, lysozyme accounts for about 169.0 μg/g of wet lung weight and 0.3% of the soluble proteins in lung homogenate. Lysozyme constitutes about 6.6% of the total soluble proteins in rat lung lavage. Metabolic labeling and immunoprecipitation were used to demonstrate that rat type II pneumocytes synthesize and secrete lysozyme in vitro. However, in human lungs, lysozyme was identified in serous submucosal glands but not in alveolar type II pneumocytes. The results demonstrate differential distribution of a secretory protein in rodent and human lungs and indicate that in the rat lysozyme could be used as an immunohistologic marker for type II pneumocytes and as an indicator of secretory activity and maturation of type II pneumocytes.

Original languageEnglish (US)
Pages (from-to)1261-1267
Number of pages7
JournalAmerican Review of Respiratory Disease
Volume138
Issue number5
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Alveolar Epithelial Cells
Muramidase
Lung
Proteins
Bronchoalveolar Lavage
Protein Sequence Analysis
Alveolar Macrophages
Pulmonary Edema
Immunoassay
Immunoprecipitation
Gestational Age
Immune Sera
Rodentia
Immunohistochemistry
Staining and Labeling
Weights and Measures
Pregnancy

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Pulmonary lysozyme - A secretory protein of type II pneumocytes in the rat. / Singh, Gurmukh; Katyal, S. L.; Brown, W. E.; Collins, D. L.; Mason, R. J.

In: American Review of Respiratory Disease, Vol. 138, No. 5, 01.01.1988, p. 1261-1267.

Research output: Contribution to journalArticle

Singh, Gurmukh ; Katyal, S. L. ; Brown, W. E. ; Collins, D. L. ; Mason, R. J. / Pulmonary lysozyme - A secretory protein of type II pneumocytes in the rat. In: American Review of Respiratory Disease. 1988 ; Vol. 138, No. 5. pp. 1261-1267.
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