Purification and characterization of a human brain galectin-1 ligand

Ahmed Chadli, Jean Pierre LeCaer, Dominique Bladier, Raymonde Joubert-Caron, Michel Caron

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Our previous studies have characterized an endogenous lectin from human brain identified as galectin-1. A soluble ligand of galectin-1 was purified from human brain by affinity chromatography and preparative electrophoresis. The purified ligand (termed HBGp82, for human brain galectin-1-binding polypeptide of 82,000 daltons) has an apparent molecular mass of 82 kDa and is glycosylated by N-linked biantennary complex structures. HBGp82 was partially characterized by microsequencing of peptide fragments. Similar peptides were found in a heat shock of protein of 90,000 daltons, hsp90. However, comparison of apparent molecular weights and matrix-assisted laser desorption mass spectrometry clearly showed that HBGp82 differs to some degree from hsp90.

Original languageEnglish (US)
Pages (from-to)1640-1647
Number of pages8
JournalJournal of Neurochemistry
Volume68
Issue number4
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Galectin 1
Purification
Brain
Ligands
HSP90 Heat-Shock Proteins
Affinity chromatography
Peptides
Peptide Fragments
Molecular mass
Electrophoresis
Affinity Chromatography
Lectins
Mass spectrometry
Desorption
Mass Spectrometry
Lasers
Molecular Weight
Molecular weight
human LGALS1 protein

Keywords

  • Affinity chromatography
  • Central nervous system
  • Galectin
  • Heat shock protein
  • Human brain
  • Lectin

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Chadli, A., LeCaer, J. P., Bladier, D., Joubert-Caron, R., & Caron, M. (1997). Purification and characterization of a human brain galectin-1 ligand. Journal of Neurochemistry, 68(4), 1640-1647.

Purification and characterization of a human brain galectin-1 ligand. / Chadli, Ahmed; LeCaer, Jean Pierre; Bladier, Dominique; Joubert-Caron, Raymonde; Caron, Michel.

In: Journal of Neurochemistry, Vol. 68, No. 4, 01.01.1997, p. 1640-1647.

Research output: Contribution to journalArticle

Chadli, A, LeCaer, JP, Bladier, D, Joubert-Caron, R & Caron, M 1997, 'Purification and characterization of a human brain galectin-1 ligand', Journal of Neurochemistry, vol. 68, no. 4, pp. 1640-1647.
Chadli A, LeCaer JP, Bladier D, Joubert-Caron R, Caron M. Purification and characterization of a human brain galectin-1 ligand. Journal of Neurochemistry. 1997 Jan 1;68(4):1640-1647.
Chadli, Ahmed ; LeCaer, Jean Pierre ; Bladier, Dominique ; Joubert-Caron, Raymonde ; Caron, Michel. / Purification and characterization of a human brain galectin-1 ligand. In: Journal of Neurochemistry. 1997 ; Vol. 68, No. 4. pp. 1640-1647.
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