Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides

Gregg T. Nagle, Walter R.A. Van Heumen, Mahdy A. El-Hamzawy, Alexander Kurosky

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A purification scheme is described for the isolation of secretory granules containing egg-laying prohormone-related peptides from the atrial gland of Aplysia californica, an exocrine organ in the reproductive tract. Granules were purified by differential centrifugation of atrial gland homogenates followed by centrifugation on continuous Percoll-sucrose gradients. Quantitative enzyme assays in conjunction with electron microscopic analyses demonstrated that secretory granules thus isolated were significantly purified with respect to other subcellular organelles such as mitochondria and lysosomes. Immunoelectron microscopy demonstrated that the majority (∼85%) of the purified secretory granules were immunoreactive for A-NTP (N-terminal peptide), a cleavage product of the egg-laying prohormone-related A and A′ precursors (residues 22-34). The purified granules represented an enriched source of peptides that were readily resolved by reversed-phase high performance liquid chromatography.

Original languageEnglish (US)
Pages (from-to)101-108
Number of pages8
JournalPeptides
Volume15
Issue number1
DOIs
StatePublished - Jan 1994

Fingerprint

Aplysia
Centrifugation
Secretory Vesicles
Purification
Ovum
Peptides
Mitochondria
High performance liquid chromatography
Sucrose
Assays
Microscopic examination
Immunoelectron Microscopy
Enzyme Assays
Reverse-Phase Chromatography
Lysosomes
Organelles
Electrons
Enzymes
High Pressure Liquid Chromatography
Mollusca egg-laying hormone

Keywords

  • Aplysia californica
  • Atrial gland
  • Egg-laying hormone gene family

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides. / Nagle, Gregg T.; Van Heumen, Walter R.A.; El-Hamzawy, Mahdy A.; Kurosky, Alexander.

In: Peptides, Vol. 15, No. 1, 01.1994, p. 101-108.

Research output: Contribution to journalArticle

Nagle, Gregg T. ; Van Heumen, Walter R.A. ; El-Hamzawy, Mahdy A. ; Kurosky, Alexander. / Purification and characterization of Aplysia atrial gland secretory granules containing egg-laying prohormone-related peptides. In: Peptides. 1994 ; Vol. 15, No. 1. pp. 101-108.
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