Purification and cytotoxic potential of myeloperoxidase in cystic fibrosis sputum

Jeannette R. Mohammed, B. Selma Mohammed, Lew J. Pawluk, Donna M. Bucci, Neil R. Baker, W. Bruce Davis

Research output: Contribution to journalArticle

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Abstract

The neutrophil myeloperoxidose-H2O2-halide enzyme system produces hypochlorous acid and chlorinated amine compounds capable of killing a variety of target cells. In the present study we hypothesized that the myeloperoxidase enzyme system is one mechanism for airway epithelial damage in patients with cystic fibrosis (CF). Enzyme linked Immunosorbent assay detected high antigenic levels of myeloperoxidase in sputum samples of seven patients with CF. Myeloperoxidase was purified to homogeneity from CF sputum and from blood neutrophils by a three-step technique involving dialysis, gel filtration, and ion-exchange chromatography. CF sputum myeloperoxidose and neutrophil myeloperoxidase appeared identical by acid gel electrophoreses and Ouchteriony experiments. CF sputum myeloperoxidase also contained approximately the same enzymatic activity as neutrophil myeloperoxidase. The myeloperoxidase enzyme system was tested for its cytotoxic potential in a tracheal ring culture system. Myeloperoxidase-induced cytotoxicity for airway epithelium was confirmed by light microscopy and rodiolabeling experiments. These findings suggest a possible role for neutrophil myeloperoxidose in CF lung disease.

Original languageEnglish (US)
Pages (from-to)711-720
Number of pages10
JournalThe Journal of Laboratory and Clinical Medicine
Volume112
Issue number6
StatePublished - Jan 1 1988

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Sputum
Cystic Fibrosis
Peroxidase
Purification
Neutrophils
Enzymes
Gels
Hypochlorous Acid
Immunosorbents
Pulmonary diseases
Dialysis
Ion Exchange Chromatography
Cytotoxicity
Chromatography
Cell culture
Lung Diseases
Optical microscopy
Amines
Gel Chromatography
Microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Mohammed, J. R., Selma Mohammed, B., Pawluk, L. J., Bucci, D. M., Baker, N. R., & Bruce Davis, W. (1988). Purification and cytotoxic potential of myeloperoxidase in cystic fibrosis sputum. The Journal of Laboratory and Clinical Medicine, 112(6), 711-720.

Purification and cytotoxic potential of myeloperoxidase in cystic fibrosis sputum. / Mohammed, Jeannette R.; Selma Mohammed, B.; Pawluk, Lew J.; Bucci, Donna M.; Baker, Neil R.; Bruce Davis, W.

In: The Journal of Laboratory and Clinical Medicine, Vol. 112, No. 6, 01.01.1988, p. 711-720.

Research output: Contribution to journalArticle

Mohammed, JR, Selma Mohammed, B, Pawluk, LJ, Bucci, DM, Baker, NR & Bruce Davis, W 1988, 'Purification and cytotoxic potential of myeloperoxidase in cystic fibrosis sputum', The Journal of Laboratory and Clinical Medicine, vol. 112, no. 6, pp. 711-720.
Mohammed, Jeannette R. ; Selma Mohammed, B. ; Pawluk, Lew J. ; Bucci, Donna M. ; Baker, Neil R. ; Bruce Davis, W. / Purification and cytotoxic potential of myeloperoxidase in cystic fibrosis sputum. In: The Journal of Laboratory and Clinical Medicine. 1988 ; Vol. 112, No. 6. pp. 711-720.
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