Purification and transplantation of myogenic progenitor cell derived exosomes to improve cardiac function in duchenne muscular dystrophic mice

Xuan Su, Yan Shen, Yue Jin, Meng Jiang, Neal Weintraub, Yaoliang Tang

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Duchene Muscular Dystrophy (DMD) is an X-linked recessive genetic disease caused by a lack of functional dystrophin protein. The disease cannot be cured, and as the disease progresses, the patient develops symptoms of dilated cardiomyopathy, arrhythmia, and congestive heart failure. The DMD MDX mutant mice do not express dystrophin, and are commonly used as a mouse model of DMD. In our recent study, we observed that intramyocardial injection of wide type (WT)-myogenic progenitor cells-derived exosomes (MPC-Exo) transiently restored the expression of dystrophin in the myocardium of DMD MDX mutant mice, which was associated with a transient improvement in cardiac function suggesting that WT-MPC-Exo may provide an option to relieve the cardiac symptoms of DMD. This article describes the technique of MPC-Exo purification and transplantation into hearts of DMD MDX mutant mice.

Original languageEnglish (US)
Article numbere59320
JournalJournal of Visualized Experiments
Volume2019
Issue number146
DOIs
StatePublished - Apr 2019

Keywords

  • Duchenne muscular dystrophy
  • Echocardiography
  • Exosome
  • Exosome Transplantation
  • Genetics
  • Heart function
  • Issue 146
  • Myogenic progenitor cell
  • Sequential ultracentrifugation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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