Purification, structural characterization, and myotropic activity of endothelin from trout, Oncorhynchus mykiss

Yuqi Wang, Kenneth R. Olson, Michael P. Smith, Michael James Russell, J. Michael Conlon

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Endothelin (ET) from a nontetrapod species has never been characterized, either structurally or biologically. A single molecular form of trout ET with 21-amino-acid residues was isolated in pure form from an extract of the kidney of the steelhead trout, Oncorhynchus mykiss and its primary structure established as Cys-Ser-Cys-Ala-Thr-Phe-Leu-Asp-Lys-Glu10-Cys-Val-Tyr-Phe- Cys-His-Leu-Asp-Ile-Ile20-Trp. This amino acid sequence shows only three substitutions (Ala4→Ser, Thr5→Ser, and Phe6→Trp) compared with human ET-2, demonstrating that the structure of the peptide has been well conserved during evolution and that the pathway of posttranslational processing of preproendothelin in the trout is probably similar to that in mammals. Synthetic trout ET produced concentration-dependent constrictions of isolated rings of vascular tissue from trout efferent branchial artery (EBA; pD2 = 7.90 ± 0.06, n = 5), caeliacomesenteric artery (pD2 = 8.03 ± 0.04, n = 4), anterior cardinal vein (ACV; pD2 = 8.57 ± 0.25, n = 4), and rat abdominal aorta (AO; pD2 = 8.86 ± 0.08, n = 7). Trout and rat vessels were more sensitive to mammalian ET-1 than to trout ET (pD2 for human ET-1 in: EBA = 9.12 ± 0.14; ACV = 9.90 ± 0.15; AO = 8.86 ± 0.08), but there was no significant difference in the maximum tension produced by either peptide in these vessels.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume277
Issue number6 46-6
StatePublished - Dec 1 1999
Externally publishedYes

Fingerprint

Trout
Oncorhynchus mykiss
Endothelins
Endothelin-1
Arteries
Endothelin-2
Peptides
Abdominal Aorta
Constriction
Blood Vessels
Mammals
Amino Acid Sequence
Veins
Kidney
Amino Acids

Keywords

  • Peptide synthesis
  • Rat
  • Teleost
  • Vasoconstrictor

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Purification, structural characterization, and myotropic activity of endothelin from trout, Oncorhynchus mykiss. / Wang, Yuqi; Olson, Kenneth R.; Smith, Michael P.; Russell, Michael James; Conlon, J. Michael.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 277, No. 6 46-6, 01.12.1999.

Research output: Contribution to journalArticle

Wang, Yuqi ; Olson, Kenneth R. ; Smith, Michael P. ; Russell, Michael James ; Conlon, J. Michael. / Purification, structural characterization, and myotropic activity of endothelin from trout, Oncorhynchus mykiss. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 1999 ; Vol. 277, No. 6 46-6.
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