Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening

Kyung mi Kim, Djanybek M. Adyshev, Anita Kása, Evgeny A. Zemskov, Irina A. Kolosova, Csilla Csortos, Alexander D. Verin

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We have previously demonstrated that PKC-potentiated inhibitory protein of protein phosphatase-1 (CPI-17) is expressed in lung endothelium. CPI-17, a specific inhibitor of myosin light chain phosphatase (MLCP), is involved in the endothelial cytoskeletal and barrier regulation. In this paper, we report the identification of fourteen putative CPI-17 interacting proteins in the lung using BacterioMatch Two-Hybrid System. Five of them: plectin 1 isoform 1, alpha II spectrin, OK/SW-CL.16, gelsolin isoform a, and junction plakoglobin are involved in actin cytoskeleton organization and cell adhesion, suggesting possible significance of these binding partners in CPI-17-mediated cytoskeletal reorganization of endothelial cells. Furthermore, we confirmed the specific interaction between plakoglobin and CPI-17, which is affected by the phosphorylation status of CPI-17 in human lung microvascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalMicrovascular Research
Volume88
DOIs
StatePublished - Jul 2013

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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