Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening

Kyung mi Kim, Djanybek M. Adyshev, Anita Kása, Evgeny Alexandrovich Zemskov, Irina A. Kolosova, Csilla Csortos, Alexander Dmitriyevich Verin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have previously demonstrated that PKC-potentiated inhibitory protein of protein phosphatase-1 (CPI-17) is expressed in lung endothelium. CPI-17, a specific inhibitor of myosin light chain phosphatase (MLCP), is involved in the endothelial cytoskeletal and barrier regulation. In this paper, we report the identification of fourteen putative CPI-17 interacting proteins in the lung using BacterioMatch Two-Hybrid System. Five of them: plectin 1 isoform 1, alpha II spectrin, OK/SW-CL.16, gelsolin isoform a, and junction plakoglobin are involved in actin cytoskeleton organization and cell adhesion, suggesting possible significance of these binding partners in CPI-17-mediated cytoskeletal reorganization of endothelial cells. Furthermore, we confirmed the specific interaction between plakoglobin and CPI-17, which is affected by the phosphorylation status of CPI-17 in human lung microvascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalMicrovascular Research
Volume88
DOIs
StatePublished - Jul 1 2013

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Screening
gamma Catenin
Proteins
Endothelial cells
Lung
Protein Isoforms
Plectin
Endothelial Cells
Myosin-Light-Chain Phosphatase
Gelsolin
Protein Phosphatase 1
Spectrin
Phosphorylation
Cell adhesion
Hybrid systems
Actin Cytoskeleton
Cell Adhesion
Endothelium
cyclopropapyrroloindole
human PPP1R14A protein

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening. / Kim, Kyung mi; Adyshev, Djanybek M.; Kása, Anita; Zemskov, Evgeny Alexandrovich; Kolosova, Irina A.; Csortos, Csilla; Verin, Alexander Dmitriyevich.

In: Microvascular Research, Vol. 88, 01.07.2013, p. 19-24.

Research output: Contribution to journalArticle

Kim, Kyung mi ; Adyshev, Djanybek M. ; Kása, Anita ; Zemskov, Evgeny Alexandrovich ; Kolosova, Irina A. ; Csortos, Csilla ; Verin, Alexander Dmitriyevich. / Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening. In: Microvascular Research. 2013 ; Vol. 88. pp. 19-24.
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AU - Verin, Alexander Dmitriyevich

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