PYK2/PDZ-RhoGEF links Ca2+ signaling to RhoA

Zhekang Ying, Fernanda R.C. Giachini, Rita C. Tostes, R. Clinton Webb

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

OBJECTIVE-: Ras homolog gene family member A (RhoA)/Rho-kinase-mediated Ca sensitization is a critical component of constrictor responses. The present study investigates how angiotensin II activates RhoA. METHODS AND RESULTS-: Adenoviral vectors were used to manipulate the expression of regulator of G protein signaling (RGS) domain containing Rho-specific guanine exchange factors (RhoGEFs) and proline-rich tyrosine kinase 2 (PYK2), a nonreceptor tyrosine kinase, in primary rat vascular smooth muscle cells. As an evidence of RhoA activation, RhoA translocation and MYPT1 (the regulatory subunit of myosin light chain phosphatase) phosphorylation were analyzed by Western blot. Results showed that overexpression of PDZ-RhoGEF, but not p115-RhoGEF or leukemia-associated RhoGEF (LARG), enhanced RhoA activation by angiotensin II. Knockdown of PDZ-RhoGEF decreased RhoA activation by angiotensin II. PDZ-RhoGEF was phosphorylated and activated by PYK2 in vitro, and knockdown of PDZ-RhoGEF reduced RhoA activation by constitutively active PYK2, indicating that PDZ-RhoGEF links PYK2 to RhoA. Knockdown of PYK2 or PDZ-RhoGEF markedly decreased RhoA activation by A23187, a Ca ionophore, demonstrating that PYK2/PDZ-RhoGEF couples RhoA activation to Ca. CONCLUSIONS-: PYK2 and PDZ-RhoGEF are necessary for angiotensin II-induced RhoA activation and for Ca signaling to RhoA.

Original languageEnglish (US)
Pages (from-to)1657-1663
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume29
Issue number10
DOIs
StatePublished - Oct 1 2009

Fingerprint

Focal Adhesion Kinase 2
Guanine
Angiotensin II
Myosin-Light-Chain Phosphatase
rho-Associated Kinases
ras Genes
Ionophores
Calcimycin
Vascular Smooth Muscle
GTP-Binding Proteins
Protein-Tyrosine Kinases
Smooth Muscle Myocytes

Keywords

  • Angiotensin II
  • Ca sensitization
  • PDZ-RhoGEF
  • PYK2
  • RhoA

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

PYK2/PDZ-RhoGEF links Ca2+ signaling to RhoA. / Ying, Zhekang; Giachini, Fernanda R.C.; Tostes, Rita C.; Webb, R. Clinton.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, No. 10, 01.10.2009, p. 1657-1663.

Research output: Contribution to journalArticle

Ying, Zhekang ; Giachini, Fernanda R.C. ; Tostes, Rita C. ; Webb, R. Clinton. / PYK2/PDZ-RhoGEF links Ca2+ signaling to RhoA. In: Arteriosclerosis, thrombosis, and vascular biology. 2009 ; Vol. 29, No. 10. pp. 1657-1663.
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abstract = "OBJECTIVE-: Ras homolog gene family member A (RhoA)/Rho-kinase-mediated Ca sensitization is a critical component of constrictor responses. The present study investigates how angiotensin II activates RhoA. METHODS AND RESULTS-: Adenoviral vectors were used to manipulate the expression of regulator of G protein signaling (RGS) domain containing Rho-specific guanine exchange factors (RhoGEFs) and proline-rich tyrosine kinase 2 (PYK2), a nonreceptor tyrosine kinase, in primary rat vascular smooth muscle cells. As an evidence of RhoA activation, RhoA translocation and MYPT1 (the regulatory subunit of myosin light chain phosphatase) phosphorylation were analyzed by Western blot. Results showed that overexpression of PDZ-RhoGEF, but not p115-RhoGEF or leukemia-associated RhoGEF (LARG), enhanced RhoA activation by angiotensin II. Knockdown of PDZ-RhoGEF decreased RhoA activation by angiotensin II. PDZ-RhoGEF was phosphorylated and activated by PYK2 in vitro, and knockdown of PDZ-RhoGEF reduced RhoA activation by constitutively active PYK2, indicating that PDZ-RhoGEF links PYK2 to RhoA. Knockdown of PYK2 or PDZ-RhoGEF markedly decreased RhoA activation by A23187, a Ca ionophore, demonstrating that PYK2/PDZ-RhoGEF couples RhoA activation to Ca. CONCLUSIONS-: PYK2 and PDZ-RhoGEF are necessary for angiotensin II-induced RhoA activation and for Ca signaling to RhoA.",
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T1 - PYK2/PDZ-RhoGEF links Ca2+ signaling to RhoA

AU - Ying, Zhekang

AU - Giachini, Fernanda R.C.

AU - Tostes, Rita C.

AU - Webb, R. Clinton

PY - 2009/10/1

Y1 - 2009/10/1

N2 - OBJECTIVE-: Ras homolog gene family member A (RhoA)/Rho-kinase-mediated Ca sensitization is a critical component of constrictor responses. The present study investigates how angiotensin II activates RhoA. METHODS AND RESULTS-: Adenoviral vectors were used to manipulate the expression of regulator of G protein signaling (RGS) domain containing Rho-specific guanine exchange factors (RhoGEFs) and proline-rich tyrosine kinase 2 (PYK2), a nonreceptor tyrosine kinase, in primary rat vascular smooth muscle cells. As an evidence of RhoA activation, RhoA translocation and MYPT1 (the regulatory subunit of myosin light chain phosphatase) phosphorylation were analyzed by Western blot. Results showed that overexpression of PDZ-RhoGEF, but not p115-RhoGEF or leukemia-associated RhoGEF (LARG), enhanced RhoA activation by angiotensin II. Knockdown of PDZ-RhoGEF decreased RhoA activation by angiotensin II. PDZ-RhoGEF was phosphorylated and activated by PYK2 in vitro, and knockdown of PDZ-RhoGEF reduced RhoA activation by constitutively active PYK2, indicating that PDZ-RhoGEF links PYK2 to RhoA. Knockdown of PYK2 or PDZ-RhoGEF markedly decreased RhoA activation by A23187, a Ca ionophore, demonstrating that PYK2/PDZ-RhoGEF couples RhoA activation to Ca. CONCLUSIONS-: PYK2 and PDZ-RhoGEF are necessary for angiotensin II-induced RhoA activation and for Ca signaling to RhoA.

AB - OBJECTIVE-: Ras homolog gene family member A (RhoA)/Rho-kinase-mediated Ca sensitization is a critical component of constrictor responses. The present study investigates how angiotensin II activates RhoA. METHODS AND RESULTS-: Adenoviral vectors were used to manipulate the expression of regulator of G protein signaling (RGS) domain containing Rho-specific guanine exchange factors (RhoGEFs) and proline-rich tyrosine kinase 2 (PYK2), a nonreceptor tyrosine kinase, in primary rat vascular smooth muscle cells. As an evidence of RhoA activation, RhoA translocation and MYPT1 (the regulatory subunit of myosin light chain phosphatase) phosphorylation were analyzed by Western blot. Results showed that overexpression of PDZ-RhoGEF, but not p115-RhoGEF or leukemia-associated RhoGEF (LARG), enhanced RhoA activation by angiotensin II. Knockdown of PDZ-RhoGEF decreased RhoA activation by angiotensin II. PDZ-RhoGEF was phosphorylated and activated by PYK2 in vitro, and knockdown of PDZ-RhoGEF reduced RhoA activation by constitutively active PYK2, indicating that PDZ-RhoGEF links PYK2 to RhoA. Knockdown of PYK2 or PDZ-RhoGEF markedly decreased RhoA activation by A23187, a Ca ionophore, demonstrating that PYK2/PDZ-RhoGEF couples RhoA activation to Ca. CONCLUSIONS-: PYK2 and PDZ-RhoGEF are necessary for angiotensin II-induced RhoA activation and for Ca signaling to RhoA.

KW - Angiotensin II

KW - Ca sensitization

KW - PDZ-RhoGEF

KW - PYK2

KW - RhoA

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