Quantification of hemoglobins S, C, and F by a magnetic affinity immunoassay

H. Moscoso, C. R. Kiefer, Abdullah Kutlar, F. A. Garver

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This magnetic affinity immunoassay (MAIA) quantifies hemoglobins (Hb), S, C, and F in hemolysates from adults or newborns. Monospecific antisera to the hemoglobins are covalently conjugated to magnetic beads and reacted with the corresponding 125I-labeled hemoglobin. After centrifugation to separate the free and antibody-bound 125I-labeled hemoglobin, the amount of radioactive hemoglobin in the pellet is measured. To determine the concentration of the Hb under study, the percent inhibition of the reaction is quantified. The standard curve is established by adding known quantities of unlabeled hemoglobin before adding 125I-labeled hemoglobin. The amount of Hb, S, Hb C, or Hb F present in hemolysates is determined by measuring the percentage of inhibition and extrapolating the concentration from the standard curve. Results agree well with values for Hb S and Hb C obtained by 'high-performance' liquid chromatography and RIA and for Hb F as measured by alkali denaturation and RIA. This assay can be completed in 1 h and is more sensitive than enzyme immunoassay or RIA: we can detect proportions of Hb S and Hb C as low as % of total Hb, and Hb F as low as 0.05%.

Original languageEnglish (US)
Pages (from-to)902-905
Number of pages4
JournalClinical Chemistry
Volume34
Issue number5
StatePublished - Jan 1 1988

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Hemoglobin C
Sickle Hemoglobin
Fetal Hemoglobin
Immunoassay
Hemoglobins
Denaturation
Centrifugation
High performance liquid chromatography
Alkalies
Immunoenzyme Techniques
Immune Sera
Assays
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Moscoso, H., Kiefer, C. R., Kutlar, A., & Garver, F. A. (1988). Quantification of hemoglobins S, C, and F by a magnetic affinity immunoassay. Clinical Chemistry, 34(5), 902-905.

Quantification of hemoglobins S, C, and F by a magnetic affinity immunoassay. / Moscoso, H.; Kiefer, C. R.; Kutlar, Abdullah; Garver, F. A.

In: Clinical Chemistry, Vol. 34, No. 5, 01.01.1988, p. 902-905.

Research output: Contribution to journalArticle

Moscoso, H, Kiefer, CR, Kutlar, A & Garver, FA 1988, 'Quantification of hemoglobins S, C, and F by a magnetic affinity immunoassay', Clinical Chemistry, vol. 34, no. 5, pp. 902-905.
Moscoso, H. ; Kiefer, C. R. ; Kutlar, Abdullah ; Garver, F. A. / Quantification of hemoglobins S, C, and F by a magnetic affinity immunoassay. In: Clinical Chemistry. 1988 ; Vol. 34, No. 5. pp. 902-905.
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