Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

John C. Sutherland, Denise C. Monteleone, John G. Trunk, Paula V. Bennett, Betsy M. Sutherland

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

DNA damages that can be converted to single- or doubl strand breaks ca be quntified by separating DNA y gel electrophoreis and obtinin a uantitative image of the resuling distribution o DNA in the gel. We review hetheory of this metho and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to cquired images o fluorophore labeled DNA.

Original languageEnglish (US)
Pages (from-to)843-854
Number of pages12
JournalELECTROPHORESIS
Volume22
Issue number5
DOIs
StatePublished - May 1 2001
Externally publishedYes

Fingerprint

Electrophoresis
DNA Damage
Gels
Imaging techniques
DNA
Fluorophores
CCD cameras
Equipment and Supplies

Keywords

  • Agarose gel electrophoresis
  • DNA damage and repair
  • Electronic imaging

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis. / Sutherland, John C.; Monteleone, Denise C.; Trunk, John G.; Bennett, Paula V.; Sutherland, Betsy M.

In: ELECTROPHORESIS, Vol. 22, No. 5, 01.05.2001, p. 843-854.

Research output: Contribution to journalArticle

Sutherland, John C. ; Monteleone, Denise C. ; Trunk, John G. ; Bennett, Paula V. ; Sutherland, Betsy M. / Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis. In: ELECTROPHORESIS. 2001 ; Vol. 22, No. 5. pp. 843-854.
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