Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

John C. Sutherland, Denise C. Monteleone, John G. Trunk, Paula V. Bennett, Betsy M. Sutherland

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

DNA damages that can be converted to single- or doubl strand breaks ca be quntified by separating DNA y gel electrophoreis and obtinin a uantitative image of the resuling distribution o DNA in the gel. We review hetheory of this metho and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to cquired images o fluorophore labeled DNA.

Original languageEnglish (US)
Pages (from-to)843-854
Number of pages12
JournalELECTROPHORESIS
Volume22
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Agarose gel electrophoresis
  • DNA damage and repair
  • Electronic imaging

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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