Quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet

Corey A. Cohn, Aimee Pak, Daniel Strongin, Martin A. Schoonen

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Hydrogen peroxide is present in many natural waters and wastewaters. In the presence of Fe(II), this species decomposes to form hydroxyl radicals, that are extremely reactive. Hence, in the presence of Fe(II), hydrogen peroxide is difficult to detect because of its short lifetime. Here, we show an expanded use of a hydrogen peroxide quantification technique using leuco crystal violet (LCV) for solutions of varying pH and iron concentration. In the presence of the biocatalyst peroxidase, LCV is oxidized by hydrogen peroxide, forming a colored crystal violet ion (CV+), which is stable for days. The LCV method uses standard equipment and allows for detection at the low microM concentration level. Results show strong pH dependence with maximum LCV oxidation at pH 4.23. By chelating dissolved Fe(II) with EDTA, hydrogen peroxide can be stabilized for analysis. Results are presented for hydrogen peroxide quantification in pyrite-water slurries. Pyrite-water slurries show surface area dependent generation of hydrogen peroxide only in the presence of EDTA, which chelates dissolved Fe(II). Given the stability of CV+, this method is particularly useful for field work that involves the detection of hydrogen peroxide.

Original languageEnglish (US)
Pages (from-to)47-51
Number of pages5
JournalGeochemical Transactions
Volume6
Issue number3
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

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Gentian Violet
hydrogen peroxide
Hydrogen Peroxide
Iron
crystal
iron
Slurries
EDTA
Edetic Acid
Water
pyrite
chelate
hydroxyl radical
Chelation
Hydroxyl Radical
water
Peroxidase
fieldwork
Wastewater
surface area

ASJC Scopus subject areas

  • Geochemistry and Petrology

Cite this

Quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet. / Cohn, Corey A.; Pak, Aimee; Strongin, Daniel; Schoonen, Martin A.

In: Geochemical Transactions, Vol. 6, No. 3, 01.06.2005, p. 47-51.

Research output: Contribution to journalArticle

Cohn, Corey A. ; Pak, Aimee ; Strongin, Daniel ; Schoonen, Martin A. / Quantifying hydrogen peroxide in iron-containing solutions using leuco crystal violet. In: Geochemical Transactions. 2005 ; Vol. 6, No. 3. pp. 47-51.
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