TY - JOUR
T1 - Quantitation of cotinine and its metabolites in rat plasma and brain tissue by hydrophilic interaction chromatography tandem mass spectrometry (HILIC-MS/MS)
AU - Li, Pei
AU - Beck, Wayne D.
AU - Callahan, Patrick Michael
AU - Terry, Alvin V.
AU - Bartlett, Michael G.
N1 - Funding Information:
The experiments described in this manuscript were supported in part by grants from the National Institute on Aging ( AG029617 ), the National Institute on Drug Abuse ( DA029127 ), and the National Institute of Environmental Health Sciences ( ES012241 ).
PY - 2012/10/15
Y1 - 2012/10/15
N2 - In this work, we developed a sensitive method to quantify cotinine (COT), norcotinine (NCOT), trans-3'-hydroxycotinine (OHCOT) and cotinine-N-oxide (COTNO) in rat plasma and brain tissue, using solid phase extraction (SPE), hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS). The linear range was 1-100. ng/mL for each analyte in rat plasma and brain homogenate (3-300. ng/g brain tissue). The method was validated with precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE). Stable isotope-labeled internal standards (IS) were used for all the analytes to achieve good reproducibility, minimizing the influence of recovery and matrix effects. This method can be used in future studies to simultaneously determine the concentrations of COT and three major metabolites in rat plasma and brain tissue.
AB - In this work, we developed a sensitive method to quantify cotinine (COT), norcotinine (NCOT), trans-3'-hydroxycotinine (OHCOT) and cotinine-N-oxide (COTNO) in rat plasma and brain tissue, using solid phase extraction (SPE), hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS). The linear range was 1-100. ng/mL for each analyte in rat plasma and brain homogenate (3-300. ng/g brain tissue). The method was validated with precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE). Stable isotope-labeled internal standards (IS) were used for all the analytes to achieve good reproducibility, minimizing the influence of recovery and matrix effects. This method can be used in future studies to simultaneously determine the concentrations of COT and three major metabolites in rat plasma and brain tissue.
KW - Brain
KW - Cotinine
KW - Cotinine-N-oxide
KW - Hydrophilic interaction chromatography
KW - Norcotinine
KW - Plasma
KW - Tandem mass spectrometry
KW - Trans-3'-hydroxycotinine
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U2 - 10.1016/j.jchromb.2012.09.018
DO - 10.1016/j.jchromb.2012.09.018
M3 - Article
C2 - 23022114
AN - SCOPUS:84867401322
SN - 1570-0232
VL - 907
SP - 117
EP - 125
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -