Quantitation of cotinine and its metabolites in rat plasma and brain tissue by hydrophilic interaction chromatography tandem mass spectrometry (HILIC-MS/MS)

Pei Li, Wayne D. Beck, Patrick Michael Callahan, Alvin V Terry, Michael G. Bartlett

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In this work, we developed a sensitive method to quantify cotinine (COT), norcotinine (NCOT), trans-3'-hydroxycotinine (OHCOT) and cotinine-N-oxide (COTNO) in rat plasma and brain tissue, using solid phase extraction (SPE), hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS). The linear range was 1-100. ng/mL for each analyte in rat plasma and brain homogenate (3-300. ng/g brain tissue). The method was validated with precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE). Stable isotope-labeled internal standards (IS) were used for all the analytes to achieve good reproducibility, minimizing the influence of recovery and matrix effects. This method can be used in future studies to simultaneously determine the concentrations of COT and three major metabolites in rat plasma and brain tissue.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume907
DOIs
StatePublished - Oct 15 2012

Fingerprint

Cotinine
Liquid chromatography
Beam plasma interactions
Metabolites
Tandem Mass Spectrometry
Chromatography
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
Mass spectrometry
Rats
Brain
Tissue
Plasmas
Solid Phase Extraction
Isotopes
Recovery

Keywords

  • Brain
  • Cotinine
  • Cotinine-N-oxide
  • Hydrophilic interaction chromatography
  • Norcotinine
  • Plasma
  • Tandem mass spectrometry
  • Trans-3'-hydroxycotinine

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

@article{67b693c8581949818da9ae56f3a2ad63,
title = "Quantitation of cotinine and its metabolites in rat plasma and brain tissue by hydrophilic interaction chromatography tandem mass spectrometry (HILIC-MS/MS)",
abstract = "In this work, we developed a sensitive method to quantify cotinine (COT), norcotinine (NCOT), trans-3'-hydroxycotinine (OHCOT) and cotinine-N-oxide (COTNO) in rat plasma and brain tissue, using solid phase extraction (SPE), hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS). The linear range was 1-100. ng/mL for each analyte in rat plasma and brain homogenate (3-300. ng/g brain tissue). The method was validated with precision within 15{\%} relative standard deviation (RSD) and accuracy within 15{\%} relative error (RE). Stable isotope-labeled internal standards (IS) were used for all the analytes to achieve good reproducibility, minimizing the influence of recovery and matrix effects. This method can be used in future studies to simultaneously determine the concentrations of COT and three major metabolites in rat plasma and brain tissue.",
keywords = "Brain, Cotinine, Cotinine-N-oxide, Hydrophilic interaction chromatography, Norcotinine, Plasma, Tandem mass spectrometry, Trans-3'-hydroxycotinine",
author = "Pei Li and Beck, {Wayne D.} and Callahan, {Patrick Michael} and Terry, {Alvin V} and Bartlett, {Michael G.}",
year = "2012",
month = "10",
day = "15",
doi = "10.1016/j.jchromb.2012.09.018",
language = "English (US)",
volume = "907",
pages = "117--125",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",

}

TY - JOUR

T1 - Quantitation of cotinine and its metabolites in rat plasma and brain tissue by hydrophilic interaction chromatography tandem mass spectrometry (HILIC-MS/MS)

AU - Li, Pei

AU - Beck, Wayne D.

AU - Callahan, Patrick Michael

AU - Terry, Alvin V

AU - Bartlett, Michael G.

PY - 2012/10/15

Y1 - 2012/10/15

N2 - In this work, we developed a sensitive method to quantify cotinine (COT), norcotinine (NCOT), trans-3'-hydroxycotinine (OHCOT) and cotinine-N-oxide (COTNO) in rat plasma and brain tissue, using solid phase extraction (SPE), hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS). The linear range was 1-100. ng/mL for each analyte in rat plasma and brain homogenate (3-300. ng/g brain tissue). The method was validated with precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE). Stable isotope-labeled internal standards (IS) were used for all the analytes to achieve good reproducibility, minimizing the influence of recovery and matrix effects. This method can be used in future studies to simultaneously determine the concentrations of COT and three major metabolites in rat plasma and brain tissue.

AB - In this work, we developed a sensitive method to quantify cotinine (COT), norcotinine (NCOT), trans-3'-hydroxycotinine (OHCOT) and cotinine-N-oxide (COTNO) in rat plasma and brain tissue, using solid phase extraction (SPE), hydrophilic interaction liquid chromatography (HILIC) and tandem mass spectrometry (MS/MS). The linear range was 1-100. ng/mL for each analyte in rat plasma and brain homogenate (3-300. ng/g brain tissue). The method was validated with precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE). Stable isotope-labeled internal standards (IS) were used for all the analytes to achieve good reproducibility, minimizing the influence of recovery and matrix effects. This method can be used in future studies to simultaneously determine the concentrations of COT and three major metabolites in rat plasma and brain tissue.

KW - Brain

KW - Cotinine

KW - Cotinine-N-oxide

KW - Hydrophilic interaction chromatography

KW - Norcotinine

KW - Plasma

KW - Tandem mass spectrometry

KW - Trans-3'-hydroxycotinine

UR - http://www.scopus.com/inward/record.url?scp=84867401322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867401322&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2012.09.018

DO - 10.1016/j.jchromb.2012.09.018

M3 - Article

C2 - 23022114

AN - SCOPUS:84867401322

VL - 907

SP - 117

EP - 125

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -