QUANTITATION OF DNA DAMAGE IN NON‐RADIOACTIVE DNA

Betsy M. Sutherland, Giovanni Ciarrocchi, Marina Ciomei, John C. Sutherland

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Three principal methods have been developed for measuring femtomoles of damage in nanogram quantities of non‐radioactive DNA. Lesions which can be quantified include single and double strand breaks, alkali labile sites including apurinic and apyrimidinic sites, and pyrimidine dimers. The first in vitro method measures the conversion of supercoiled DNA to relaxed or linear molecules, and can detect up to four lesions per molecule. The second in vitro method (supercoil depletion) assesses the fraction of intact linear molecules of homogeneous length, and allows detection of 8 lesions/molecule. The third method, measurement of molecular length distributions of DNAs of heterogeneous length, reveals the extent of DNA damage and repair in vivo or in vitro.

Original languageEnglish (US)
Pages (from-to)391-396
Number of pages6
JournalPhotochemistry and Photobiology
Volume44
Issue number3
DOIs
StatePublished - Sep 1986
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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