Quantitation of hemoglobins Bart's, H, Portland-I, Portland-II and constant spring by anion-exchange high-performance liquid chromatography

Ferdane Kutlar, Li Hao Gu, Huaiyu Hu, Titus H.J. Huisman

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Abstract

We introduce a new high-performance liquid chromatographic procedure that uses a specific anion-exchange column for the separation of hemoglobin (Hb) Bart's (γ4), Hb H (β4), Hb Portland-I (ζ2γ2), Hb Portland-II (ζ2β2), and the abnormal Hb Constant Spring (α2 extended β2) in cord blood and adult red cell lysates. The method provides quantitative data for Hb Bart's in cord blood that correlate well with the α-globin gene status of the babies and can be used for an initial identification of α-thalassemic conditions. Quantitation of Hb Bart's from cord blood samples that are collected on filter paper and submitted as dried blood spots is unreliable. The separation of Hb H and Hb Bart's allows an evaluation of the synthesis of these two hemoglobin components in patients with Hb H disease.

Original languageEnglish (US)
Pages (from-to)265-274
Number of pages10
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume487
Issue numberC
DOIs
Publication statusPublished - 1989

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ASJC Scopus subject areas

  • Chemistry(all)

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