Quantitation of LH subunits released by rat anterior pituitary cells in primary culture

H. Edward Grotjan, Nancy W. Leveque, Albert S. Berkowitz, Brooks Allen Keel

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Immunoreactive rLH, rLHα, rLHβ and rFSH in the cell extracts and medium of rat anterior pituitary cell cultures were measured after gel filtration on Sephadex G-100 Superfine. Cell extracts contained uncombined rLHα and rLHβ present as approximately 40% and 4%, respectively, of native rLH on a molar basis. Unstimulated cultures appeared to release a large excess of rLHβ ( $ ̃2560% of rLH on a molar basis) as well as a minimum of uncombined rLHβ. Stimulation of cultures with physiological (LHRH) or non-physiological agents (A23187, elevated K+) increased the absolute but not the relative quantities of uncombined subunits released relative to those present intracellularly. Thus, rat anterior pituitary cell cultures appear to produce and release an excess of free a subunit as well as a minimum of uncombined rLHβ. However, one cannot be certain whether the uncombined rLHβ represents a pool of 'free' rLHβ or simply results from the dissociation of rLH.

Original languageEnglish (US)
Pages (from-to)121-129
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume35
Issue number2-3
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Primary Cell Culture
Cell Extracts
Cell culture
Rats
Cell Culture Techniques
Calcimycin
Gonadotropin-Releasing Hormone
Gel Chromatography
Gels
sephadex

Keywords

  • FSH
  • LH
  • LHα
  • LHβ
  • pituitary

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Quantitation of LH subunits released by rat anterior pituitary cells in primary culture. / Grotjan, H. Edward; Leveque, Nancy W.; Berkowitz, Albert S.; Keel, Brooks Allen.

In: Molecular and Cellular Endocrinology, Vol. 35, No. 2-3, 01.01.1984, p. 121-129.

Research output: Contribution to journalArticle

Grotjan, H. Edward ; Leveque, Nancy W. ; Berkowitz, Albert S. ; Keel, Brooks Allen. / Quantitation of LH subunits released by rat anterior pituitary cells in primary culture. In: Molecular and Cellular Endocrinology. 1984 ; Vol. 35, No. 2-3. pp. 121-129.
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