Quantitative contribution of the collagen network in dentin hybridization

A. John Gwinnett, Franklin Chi Meng Tay, K. Michael Pang, Stephen H Y Wei

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Purpose: To determine the quantitative contribution of dentin hybridization to bonded assembly strength and demonstrate the micromorphology of the interface with and without collagen present. Materials and Methods: Four groups of 10 molar teeth were finished to a 320 grit dentin smear layer. Two groups served as controls and two experimental groups were subjected to collagenase digestion of the collagen exposed by acid conditioning. All-Bond 2 and Amalgambond were used to bond Bisfil and Epic resin composite, respectively. Stored in water at 37°C for 2 hours the assemblies were tested in a shear mode at a crosshead speed of 5 mm/minute. Means and standard deviations were subjected to analysis for statistical significance. Twenty four teeth in four groups were examined by scanning (SEM) and transmission electron microscopy (TEM) for the relationship between resin and conditioned dentin with and without the collagen network. Results: All-Bond 2 and Amalgambond controls were 28.41 ± 3.9 and 19.04 ± 5.96 MPa, collagenase-treated groups scored 26.43 ± 2.90 and 19.70 ± 4.25 MPa respectively. No significant difference existed between the control and experimental groups. SEM showed an intertubular collager network with patent tubules and a pronounced porous, irregular dentin topography following collagen digestion. A distinct hybrid zone and tubular penetration was observed but the collagenase-treated specimens showed only resin in the tubules and their lateral extensions. TEM confirmed the absence of a distinct hybrid zone in the collagenase groups with a tight, gap-free junction between the resin and the undemineralized dentin. An electron dense zone (< 50 nm) at the leading edge of conditioning was observed for All-Bond 2 and Amalgambond groups. It was concluded that the resin-reinforced or hybridized, collagenous network does not detract from, nor contribute any significant quantitative value per se to dentin bonding with the systems tested.

Original languageEnglish (US)
Pages (from-to)140-144
Number of pages5
JournalAmerican Journal of Dentistry
Volume9
Issue number4
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Dentin
Collagen
Collagenases
Digestion
Tooth
Smear Layer
Scanning Transmission Electron Microscopy
Composite Resins
Gap Junctions
Transmission Electron Microscopy
Electrons
Control Groups
Acids
Water
Amalgambond
All-Bond 2

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Quantitative contribution of the collagen network in dentin hybridization. / Gwinnett, A. John; Tay, Franklin Chi Meng; Pang, K. Michael; Wei, Stephen H Y.

In: American Journal of Dentistry, Vol. 9, No. 4, 01.12.1996, p. 140-144.

Research output: Contribution to journalArticle

Gwinnett, A. John ; Tay, Franklin Chi Meng ; Pang, K. Michael ; Wei, Stephen H Y. / Quantitative contribution of the collagen network in dentin hybridization. In: American Journal of Dentistry. 1996 ; Vol. 9, No. 4. pp. 140-144.
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