Quantitative differential expression analysis reveals miR-7 as major islet microRNA

Valia Bravo-Egana, Samuel Rosero, R. Damaris Molano, Antonello Pileggi, Camillo Ricordi, Juan Domínguez-Bendala, Ricardo L. Pastori

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.

Original languageEnglish (US)
Pages (from-to)922-926
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number4
DOIs
StatePublished - Feb 22 2008

Keywords

  • MicroRNA microarrays
  • MicroRNAs
  • Pancreatic islets
  • q-RT-PCR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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