Quantitative differential expression analysis reveals miR-7 as major islet microRNA

Valia Bravo-Egana; Samuel Rosero; R. Damaris Molano; Antonello Pileggi; Camillo Ricordi; Juan Domínguez-Bendala; Ricardo L. Pastori

doi:10.1016/j.bbrc.2007.12.052

Quantitative differential expression analysis reveals miR-7 as major islet microRNA

Valia Bravo-Egana, Samuel Rosero, R. Damaris Molano, Antonello Pileggi, Camillo Ricordi, Juan Domínguez-Bendala, Ricardo L. Pastori

Research output: Contribution to journal › Article › peer-review

110 Scopus citations

Abstract

MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.

Original language	English (US)
Pages (from-to)	922-926
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	366
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2007.12.052
State	Published - Feb 22 2008
Externally published	Yes

Keywords

MicroRNA microarrays
MicroRNAs
Pancreatic islets
q-RT-PCR

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Quantitative differential expression analysis reveals miR-7 as major islet microRNA. / Bravo-Egana, Valia; Rosero, Samuel; Molano, R. Damaris; Pileggi, Antonello; Ricordi, Camillo; Domínguez-Bendala, Juan; Pastori, Ricardo L.

In: Biochemical and Biophysical Research Communications, Vol. 366, No. 4, 22.02.2008, p. 922-926.

Research output: Contribution to journal › Article › peer-review

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title = "Quantitative differential expression analysis reveals miR-7 as major islet microRNA",

abstract = "MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.",

keywords = "MicroRNA microarrays, MicroRNAs, Pancreatic islets, q-RT-PCR",

author = "Valia Bravo-Egana and Samuel Rosero and Molano, {R. Damaris} and Antonello Pileggi and Camillo Ricordi and Juan Dom{\'i}nguez-Bendala and Pastori, {Ricardo L.}",

note = "Funding Information: This work was supported by National Institutes of Health-NCRR-Islet Cell Resources (U42 RR016603) grant, the Diabetes Research Institute Foundation, the Peacock Foundation and the Foundation for Diabetes Research. We thank Dr. Dagmar Klein for critical reading of the manuscript and Judith Molina, Elsie Zahr and Sergio San Jose for technical assistance.",

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AU - Pileggi, Antonello

AU - Ricordi, Camillo

AU - Domínguez-Bendala, Juan

AU - Pastori, Ricardo L.

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N2 - MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.

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