Abstract
MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 922-926 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 366 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 22 2008 |
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Keywords
- MicroRNA microarrays
- MicroRNAs
- Pancreatic islets
- q-RT-PCR
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Quantitative differential expression analysis reveals miR-7 as major islet microRNA. / Bravo Egana, Valia; Rosero, Samuel; Molano, R. Damaris; Pileggi, Antonello; Ricordi, Camillo; Domínguez-Bendala, Juan; Pastori, Ricardo L.
In: Biochemical and Biophysical Research Communications, Vol. 366, No. 4, 22.02.2008, p. 922-926.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quantitative differential expression analysis reveals miR-7 as major islet microRNA
AU - Bravo Egana, Valia
AU - Rosero, Samuel
AU - Molano, R. Damaris
AU - Pileggi, Antonello
AU - Ricordi, Camillo
AU - Domínguez-Bendala, Juan
AU - Pastori, Ricardo L.
PY - 2008/2/22
Y1 - 2008/2/22
N2 - MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.
AB - MicroRNAs (miRNAs) are non-coding gene products that regulate gene expression through specific binding to target mRNAs. Cell-specific patterns of miRNAs are associated with the acquisition and maintenance of a given phenotype, such as endocrine pancreas (islets). We hypothesized that a subset of miRNAs could be differentially expressed in the islets. Using miRNA microarray technology and quantitative RT-PCR we identified a subset of miRNAs that are the most differentially expressed islet miRNAs (ratio islet/acinar > 150-fold), miR-7 being the most abundant. A similarly high ratio for miR-7 was observed in human islets. The ratio islet/acinar for miR-375, a previously described islet miRNA, was <10 and is 2.5× more abundant in the islets than miR-7. Therefore, we conclude that miR-7 is the most abundant endocrine miRNA in islets while miR-375 is the most abundant intra-islet miRNA. Our results may offer new insights into regulatory pathways of islet gene expression.
KW - MicroRNA microarrays
KW - MicroRNAs
KW - Pancreatic islets
KW - q-RT-PCR
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UR - http://www.scopus.com/inward/citedby.url?scp=37549021902&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.12.052
DO - 10.1016/j.bbrc.2007.12.052
M3 - Article
C2 - 18086561
AN - SCOPUS:37549021902
VL - 366
SP - 922
EP - 926
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -