Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling

Prithu Sundd, Edgar Gutierrez, Maria K. Pospieszalska, Hong Zhang, Alexander Groisman, Klaus Ley

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

We introduce quantitative dynamic footprinting microscopy to resolve neutrophil rolling on P-selectin. We observed that the footprint of a rolling neutrophil was fourfold larger than previously thought, and that P-selectin-PSGL-1 bonds were relaxed at the leading edge of the rolling cell, compressed under the cell center, and stretched at the trailing edge. Each rolling neutrophil formed three to four long tethers that extended up to 16 μ behind the rolling cell.

Original languageEnglish (US)
Pages (from-to)821-824
Number of pages4
JournalNature Methods
Volume7
Issue number10
DOIs
StatePublished - Oct 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling'. Together they form a unique fingerprint.

Cite this