Quantitative evaluation of microvascular density after stroke in rats using MRI

Asamoah Bosomtwi; Quan Jiang; Guang L. Ding; Li Zhang; Zheng G. Zhang; Mei Lu; James R. Ewing; Michael Chopp

doi:10.1038/jcbfm.2008.85

Quantitative evaluation of microvascular density after stroke in rats using MRI

Asamoah Bosomtwi, Quan Jiang, Guang L. Ding, Li Zhang, Zheng G. Zhang, Mei Lu, James R. Ewing, Michael Chopp

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

We investigated vascular changes after stroke using magnetic resonance imaging (MRI) microvascular density (MVD) measurement. T₂ and T ₂ were measured in eight rats before and after injecting an intravascular superparamagnetic iron oxide contrast agent to derive the corresponding transverse relaxation shift. Reliability of MRI for measurement of MVD was compared with corresponding sections immunostained with von Willebrand factor (vWF) 2 weeks after stroke. The intracorrelation coefficient (ICC) and its 95% lower bound (LB) was high in the ischemic recovery region (ICC=0.753), moderate in the contralateral area of normal brain tissue (ICC=0.70), and low in the ischemic core (ICC=0.24). A very good agreement (ICC=0.85) and correlation (r=0.90) were observed using only the recovery region and normal contralateral hemisphere (ICC=0.85; 95% LB=0.78; P<0.05). The mean MRI MVD in the center of the core lesion (26±9 per mm²) was lower than in the recovery region (209±60 per mm²) or contralateral normal hemisphere (313±32 per mm²). However, large errors in MRI MVD were encountered in the ischemic core. Our data demonstrate that MRI MVD measurements can quantitatively evaluate microvascular changes in the brain tissue after stroke, if the MVD is not extremely low as in the ischemic core.

Original language	English (US)
Pages (from-to)	1978-1987
Number of pages	10
Journal	Journal of Cerebral Blood Flow and Metabolism
Volume	28
Issue number	12
DOIs	https://doi.org/10.1038/jcbfm.2008.85
State	Published - Dec 2008
Externally published	Yes

Keywords

Angiogenesis
Cerebral ischemia
Contrast agent
MRI
Microvessel density
Stroke

ASJC Scopus subject areas

Neurology
Clinical Neurology
Cardiology and Cardiovascular Medicine

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10.1038/jcbfm.2008.85

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title = "Quantitative evaluation of microvascular density after stroke in rats using MRI",

abstract = "We investigated vascular changes after stroke using magnetic resonance imaging (MRI) microvascular density (MVD) measurement. T2 and T 2 were measured in eight rats before and after injecting an intravascular superparamagnetic iron oxide contrast agent to derive the corresponding transverse relaxation shift. Reliability of MRI for measurement of MVD was compared with corresponding sections immunostained with von Willebrand factor (vWF) 2 weeks after stroke. The intracorrelation coefficient (ICC) and its 95% lower bound (LB) was high in the ischemic recovery region (ICC=0.753), moderate in the contralateral area of normal brain tissue (ICC=0.70), and low in the ischemic core (ICC=0.24). A very good agreement (ICC=0.85) and correlation (r=0.90) were observed using only the recovery region and normal contralateral hemisphere (ICC=0.85; 95% LB=0.78; P<0.05). The mean MRI MVD in the center of the core lesion (26±9 per mm2) was lower than in the recovery region (209±60 per mm2) or contralateral normal hemisphere (313±32 per mm2). However, large errors in MRI MVD were encountered in the ischemic core. Our data demonstrate that MRI MVD measurements can quantitatively evaluate microvascular changes in the brain tissue after stroke, if the MVD is not extremely low as in the ischemic core.",

keywords = "Angiogenesis, Cerebral ischemia, Contrast agent, MRI, Microvessel density, Stroke",

author = "Asamoah Bosomtwi and Quan Jiang and Ding, {Guang L.} and Li Zhang and Zhang, {Zheng G.} and Mei Lu and Ewing, {James R.} and Michael Chopp",

year = "2008",

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doi = "10.1038/jcbfm.2008.85",

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AU - Bosomtwi, Asamoah

AU - Jiang, Quan

AU - Ding, Guang L.

AU - Zhang, Li

AU - Zhang, Zheng G.

AU - Lu, Mei

AU - Ewing, James R.

AU - Chopp, Michael

PY - 2008/12

Y1 - 2008/12

N2 - We investigated vascular changes after stroke using magnetic resonance imaging (MRI) microvascular density (MVD) measurement. T2 and T 2 were measured in eight rats before and after injecting an intravascular superparamagnetic iron oxide contrast agent to derive the corresponding transverse relaxation shift. Reliability of MRI for measurement of MVD was compared with corresponding sections immunostained with von Willebrand factor (vWF) 2 weeks after stroke. The intracorrelation coefficient (ICC) and its 95% lower bound (LB) was high in the ischemic recovery region (ICC=0.753), moderate in the contralateral area of normal brain tissue (ICC=0.70), and low in the ischemic core (ICC=0.24). A very good agreement (ICC=0.85) and correlation (r=0.90) were observed using only the recovery region and normal contralateral hemisphere (ICC=0.85; 95% LB=0.78; P<0.05). The mean MRI MVD in the center of the core lesion (26±9 per mm2) was lower than in the recovery region (209±60 per mm2) or contralateral normal hemisphere (313±32 per mm2). However, large errors in MRI MVD were encountered in the ischemic core. Our data demonstrate that MRI MVD measurements can quantitatively evaluate microvascular changes in the brain tissue after stroke, if the MVD is not extremely low as in the ischemic core.

AB - We investigated vascular changes after stroke using magnetic resonance imaging (MRI) microvascular density (MVD) measurement. T2 and T 2 were measured in eight rats before and after injecting an intravascular superparamagnetic iron oxide contrast agent to derive the corresponding transverse relaxation shift. Reliability of MRI for measurement of MVD was compared with corresponding sections immunostained with von Willebrand factor (vWF) 2 weeks after stroke. The intracorrelation coefficient (ICC) and its 95% lower bound (LB) was high in the ischemic recovery region (ICC=0.753), moderate in the contralateral area of normal brain tissue (ICC=0.70), and low in the ischemic core (ICC=0.24). A very good agreement (ICC=0.85) and correlation (r=0.90) were observed using only the recovery region and normal contralateral hemisphere (ICC=0.85; 95% LB=0.78; P<0.05). The mean MRI MVD in the center of the core lesion (26±9 per mm2) was lower than in the recovery region (209±60 per mm2) or contralateral normal hemisphere (313±32 per mm2). However, large errors in MRI MVD were encountered in the ischemic core. Our data demonstrate that MRI MVD measurements can quantitatively evaluate microvascular changes in the brain tissue after stroke, if the MVD is not extremely low as in the ischemic core.

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KW - Cerebral ischemia

KW - Contrast agent

KW - MRI

KW - Microvessel density

KW - Stroke

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Quantitative evaluation of microvascular density after stroke in rats using MRI

Abstract

Keywords

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