Quaternary ammonium silane-based antibacterial and anti-proteolytic cavity cleanser

Ya ping Gou, Ji yao Li, Mohamed M. Meghil, Christopher W Cutler, Hockin H.K. Xu, Franklin Chi Meng Tay, Li na Niu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: Secondary caries and degradation of hybrid layers are two major challenges in achieving durable resin–dentin bonds. The objectives of the present study were to investigate the effects of a 2% quaternary ammonium silane (QAS) cavity cleanser on bacteria impregnated into dentin blocks and the gelatinolytic activity of the hybrid layers. Methods: Microtensile bond strength was first performed to evaluate if the 2% QAS cavity cleanser adversely affected bond strength. For antibacterial testing, Streptococcus mutans and Actinomyces naeslundii were impregnated into dentin blocks, respectively, prior to the application of the cavity cleanser. Live/dead bacterial staining and colony-forming unit (CFU) counts were performed to evaluate their antibacterial effects. Gelatinolytic activity within the hybrid layers was directly examined using in-situ zymography. A double-fluorescence technique was used to examine interfacial permeability immediately after bonding. Results: The cavity cleanser did not adversely affect the bond strength of the adhesives tested (p > 0.05). Antibacterial testing indicated that 2% QAS significantly killed impregnated bacteria within the dentin blocks compared with control group (p < 0.05), which was comparable with the antibacterial activity of 2% chlorhexidine (p > 0.05). Hybrid layers pretreated with 2% QAS showed significant decrease in enzyme activity compared with control group. With the use of 2% QAS, relatively lower interfacial permeability was observed, compared with control group and 2% chlorhexidine (p < 0.05). Significance: The present study developed a 2% QAS cavity cleanser that possesses combined antimicrobial and anti-proteolytic activities to extend the longevity of resin–dentin bonds.

Original languageEnglish (US)
Pages (from-to)1814-1827
Number of pages14
JournalDental Materials
Volume34
Issue number12
DOIs
StatePublished - Dec 1 2018

Fingerprint

Silanes
Ammonium Compounds
Dentin
Control Groups
Permeability
Bacteria
Actinomyces
Chlorhexidine
Streptococcus mutans
Enzyme activity
Testing
Adhesives
Stem Cells
Fluorescence
Staining and Labeling
Degradation
Enzymes

Keywords

  • Antibacterial
  • Cavity cleanser
  • Endogenous dentin proteases
  • Quaternary ammonium silane
  • Resin–dentin bonds

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Quaternary ammonium silane-based antibacterial and anti-proteolytic cavity cleanser. / Gou, Ya ping; Li, Ji yao; Meghil, Mohamed M.; Cutler, Christopher W; Xu, Hockin H.K.; Tay, Franklin Chi Meng; Niu, Li na.

In: Dental Materials, Vol. 34, No. 12, 01.12.2018, p. 1814-1827.

Research output: Contribution to journalArticle

Gou, Ya ping ; Li, Ji yao ; Meghil, Mohamed M. ; Cutler, Christopher W ; Xu, Hockin H.K. ; Tay, Franklin Chi Meng ; Niu, Li na. / Quaternary ammonium silane-based antibacterial and anti-proteolytic cavity cleanser. In: Dental Materials. 2018 ; Vol. 34, No. 12. pp. 1814-1827.
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abstract = "Objective: Secondary caries and degradation of hybrid layers are two major challenges in achieving durable resin–dentin bonds. The objectives of the present study were to investigate the effects of a 2{\%} quaternary ammonium silane (QAS) cavity cleanser on bacteria impregnated into dentin blocks and the gelatinolytic activity of the hybrid layers. Methods: Microtensile bond strength was first performed to evaluate if the 2{\%} QAS cavity cleanser adversely affected bond strength. For antibacterial testing, Streptococcus mutans and Actinomyces naeslundii were impregnated into dentin blocks, respectively, prior to the application of the cavity cleanser. Live/dead bacterial staining and colony-forming unit (CFU) counts were performed to evaluate their antibacterial effects. Gelatinolytic activity within the hybrid layers was directly examined using in-situ zymography. A double-fluorescence technique was used to examine interfacial permeability immediately after bonding. Results: The cavity cleanser did not adversely affect the bond strength of the adhesives tested (p > 0.05). Antibacterial testing indicated that 2{\%} QAS significantly killed impregnated bacteria within the dentin blocks compared with control group (p < 0.05), which was comparable with the antibacterial activity of 2{\%} chlorhexidine (p > 0.05). Hybrid layers pretreated with 2{\%} QAS showed significant decrease in enzyme activity compared with control group. With the use of 2{\%} QAS, relatively lower interfacial permeability was observed, compared with control group and 2{\%} chlorhexidine (p < 0.05). Significance: The present study developed a 2{\%} QAS cavity cleanser that possesses combined antimicrobial and anti-proteolytic activities to extend the longevity of resin–dentin bonds.",
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T1 - Quaternary ammonium silane-based antibacterial and anti-proteolytic cavity cleanser

AU - Gou, Ya ping

AU - Li, Ji yao

AU - Meghil, Mohamed M.

AU - Cutler, Christopher W

AU - Xu, Hockin H.K.

AU - Tay, Franklin Chi Meng

AU - Niu, Li na

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Objective: Secondary caries and degradation of hybrid layers are two major challenges in achieving durable resin–dentin bonds. The objectives of the present study were to investigate the effects of a 2% quaternary ammonium silane (QAS) cavity cleanser on bacteria impregnated into dentin blocks and the gelatinolytic activity of the hybrid layers. Methods: Microtensile bond strength was first performed to evaluate if the 2% QAS cavity cleanser adversely affected bond strength. For antibacterial testing, Streptococcus mutans and Actinomyces naeslundii were impregnated into dentin blocks, respectively, prior to the application of the cavity cleanser. Live/dead bacterial staining and colony-forming unit (CFU) counts were performed to evaluate their antibacterial effects. Gelatinolytic activity within the hybrid layers was directly examined using in-situ zymography. A double-fluorescence technique was used to examine interfacial permeability immediately after bonding. Results: The cavity cleanser did not adversely affect the bond strength of the adhesives tested (p > 0.05). Antibacterial testing indicated that 2% QAS significantly killed impregnated bacteria within the dentin blocks compared with control group (p < 0.05), which was comparable with the antibacterial activity of 2% chlorhexidine (p > 0.05). Hybrid layers pretreated with 2% QAS showed significant decrease in enzyme activity compared with control group. With the use of 2% QAS, relatively lower interfacial permeability was observed, compared with control group and 2% chlorhexidine (p < 0.05). Significance: The present study developed a 2% QAS cavity cleanser that possesses combined antimicrobial and anti-proteolytic activities to extend the longevity of resin–dentin bonds.

AB - Objective: Secondary caries and degradation of hybrid layers are two major challenges in achieving durable resin–dentin bonds. The objectives of the present study were to investigate the effects of a 2% quaternary ammonium silane (QAS) cavity cleanser on bacteria impregnated into dentin blocks and the gelatinolytic activity of the hybrid layers. Methods: Microtensile bond strength was first performed to evaluate if the 2% QAS cavity cleanser adversely affected bond strength. For antibacterial testing, Streptococcus mutans and Actinomyces naeslundii were impregnated into dentin blocks, respectively, prior to the application of the cavity cleanser. Live/dead bacterial staining and colony-forming unit (CFU) counts were performed to evaluate their antibacterial effects. Gelatinolytic activity within the hybrid layers was directly examined using in-situ zymography. A double-fluorescence technique was used to examine interfacial permeability immediately after bonding. Results: The cavity cleanser did not adversely affect the bond strength of the adhesives tested (p > 0.05). Antibacterial testing indicated that 2% QAS significantly killed impregnated bacteria within the dentin blocks compared with control group (p < 0.05), which was comparable with the antibacterial activity of 2% chlorhexidine (p > 0.05). Hybrid layers pretreated with 2% QAS showed significant decrease in enzyme activity compared with control group. With the use of 2% QAS, relatively lower interfacial permeability was observed, compared with control group and 2% chlorhexidine (p < 0.05). Significance: The present study developed a 2% QAS cavity cleanser that possesses combined antimicrobial and anti-proteolytic activities to extend the longevity of resin–dentin bonds.

KW - Antibacterial

KW - Cavity cleanser

KW - Endogenous dentin proteases

KW - Quaternary ammonium silane

KW - Resin–dentin bonds

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DO - 10.1016/j.dental.2018.10.001

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