Quercetin protects against oxidative stress associated damages in a rat model of transient focal cerebral ischemia and reperfusion

Ajmal Ahmad, Mohd Moshahid Khan, Md Nasrul Hoda, Syed Shadab Raza, M. Badruzzaman Khan, Hayate Javed, Tauheed Ishrat, Mohammad Ashafaq, Md Ejaz Ahmad, Mohammed M. Safhi, Fakhrul Islam

Research output: Contribution to journalArticle

71 Scopus citations

Abstract

Experimental studies have demonstrated that oxidative stress and apoptosis play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. The purpose of this study was to determine whether the quercetin dihydrate (Q) protects against cerebral ischemia neuronal damage. Male Wistar rats were subjected to transient middle cerebral artery occlusion (MCAO) for 2 h and reperfused for 72 h. Quercetin (30 mg/kg, i.p) was administrated 30 min before the onset of ischemia and after the ischemia at interval of 0, 24, 48, and 72 h. The administration of Q showed marked reduction in infarct size, reduced the neurological deficits in terms of behaviors, suppressed neuronal loss and diminished the p53 expression in MCAO rats. Q was found to be successful in upregulating the antioxidant status and lowering the TBARS level. Conversely, the elevated activity of poly (ADP-ribose) polymerase (PARP), and activity of caspase-3 in MCAO group was attenuated significantly in Q treated group when compared with MCAO group. Our study reveals that Q, as a powerful antioxidant, could prevent free radicals associated oxidative damage and morphological changes in the MCAO rats. Thus, it may have a therapeutic value for the treatment of stroke.

Original languageEnglish (US)
Pages (from-to)1360-1371
Number of pages12
JournalNeurochemical Research
Volume36
Issue number8
DOIs
StatePublished - Aug 1 2011

Keywords

  • Infarction
  • Middle cerebral artery occlusion
  • Neurobehavioral
  • Neuroprotection
  • Oxidative stress
  • Quercetin dihydrate

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Ahmad, A., Khan, M. M., Hoda, M. N., Raza, S. S., Khan, M. B., Javed, H., Ishrat, T., Ashafaq, M., Ahmad, M. E., Safhi, M. M., & Islam, F. (2011). Quercetin protects against oxidative stress associated damages in a rat model of transient focal cerebral ischemia and reperfusion. Neurochemical Research, 36(8), 1360-1371. https://doi.org/10.1007/s11064-011-0458-6