Rab 11 is an apically located small GTP-binding protein in epithelial tissues

James R. Goldenring, Jenetta Smith, Hilary D. Vaughan, Patricia L Cameron, Wendy Hawkins, Jennifer Navarre

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Rab proteins are involved in many aspects of dynamic vesicle processing within eukaryotic cells. We have previously identified Rab 11 in gastric parietal cell tubulovesicle membranes. We have produced a monoclonal antibody that is specific for Eabll. In all rabbit tissues examined, Rab 11 immunoreactivity was highly enriched in epithelial cells. In the gastric fundus, parietal cells were stained in a pattern consistent with localization on tubulovesicles. Surface mucous cells of both the fundus and antrum demonstrated punctate subapical staining. Ileal and proximal colonie enterocyte labeling was observed deep to the brush borders. In the distal colon, staining was observed in the apical regions of mid-crypt cells. In skin and esophagus, punctate immunoreactivity was present in the medial layers of the squamous epithelia. Prominent Rab 11 immunoreactivity was present in hepatocytes deep to the bile canaliculi. Punctate subapical staining was observed in pancreatic acinar cells. Apical staining was also observed in collecting duct cells and in the glandular cells of the prostate. These results indicate that Rab 11 is expressed in apical vesicular populations in discrete epithelial cell populations.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume270
Issue number3 33-3
StatePublished - Dec 1 1996

Fingerprint

GTP-Binding Proteins
Epithelium
Staining and Labeling
Epithelial Cells
Bile Canaliculi
Gastric Parietal Cells
Gastric Fundus
Enterocytes
Acinar Cells
Eukaryotic Cells
Microvilli
Population
Esophagus
Prostate
Hepatocytes
Colon
Monoclonal Antibodies
Cell Membrane
Rabbits
Skin

Keywords

  • Parietal cells
  • Rab proteins
  • Small guanosine triphosphate-binding proteins

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Rab 11 is an apically located small GTP-binding protein in epithelial tissues. / Goldenring, James R.; Smith, Jenetta; Vaughan, Hilary D.; Cameron, Patricia L; Hawkins, Wendy; Navarre, Jennifer.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 270, No. 3 33-3, 01.12.1996.

Research output: Contribution to journalArticle

Goldenring, James R. ; Smith, Jenetta ; Vaughan, Hilary D. ; Cameron, Patricia L ; Hawkins, Wendy ; Navarre, Jennifer. / Rab 11 is an apically located small GTP-binding protein in epithelial tissues. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 1996 ; Vol. 270, No. 3 33-3.
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