Rab1 GTPase promotes expression of β-adrenergic receptors in rat pulmonary microvascular endothelial cells

Yuncheng Li, Guansong Wang, Kexiong Lin, Hongjin Yin, Changxi Zhou, Ting Liu, Guangyu Wu, Guisheng Qian

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

It is known that Rab1 regulates the expression and function of beta-adrenoceptors (β-ARs) in many cells. However, the effect of these changes in rat pulmonary microvascular endothelial cells (RPMVECs) is not known. In the present study, we investigated the role of Rab1, a Ras-like GTPase that coordinates protein transport from the endoplasmic reticulum (ER) to the Golgi body and regulates the cell-surface targeting and function of endogenous β-ARs in RPMVECs in the presence of lipopolysaccharide (LPS). We found that lentivirus-driven expression of wild-type Rab1 (Rab1WT) in RPMVECs strongly enhanced the amount of β-ARs on the cell surface, whereas the dominant-negative mutant Rab1N124I significantly attenuated β-ARs expression on the cell surface. In addition, LPS stimulation significantly reduced β-ARs expression on the cell surface in RPMVECs; however, this effect was reversed by over-expression of wild-type Rab1WT. Fluorescent microscopy analysis demonstrated that expression of Rab1N124I and Rab1 small interfering RNA (siRNA) significantly induced the accumulation of green fluorescent protein (GFP)-tagged β2-AR in the ER. Consistent with their effects on β-ARs export, Rab1WT and Rab1N124I differentially modified the β-AR-mediated activation of extracellular signal-regulated kinase1/2 (ERK1/2). Importantly, over-expression of Rab1WT markedly reduced LPS-induced hyper-permeability of RPMVECs by increasing the expression of β2-AR on the cell surface. These data reveal that β-ARs function in RPMVECs could be modulated by manipulating β-ARs traffic from the ER to the Golgi body. We propose the ER-to-Golgi transport as a regulatory site for control of permeability of RPMVECs.

Original languageEnglish (US)
Pages (from-to)1201-1209
Number of pages9
JournalInternational Journal of Biochemistry and Cell Biology
Volume42
Issue number7
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Fingerprint

GTP Phosphohydrolases
Endothelial cells
Adrenergic Receptors
Rats
Endothelial Cells
Lung
Endoplasmic Reticulum
Lipopolysaccharides
Permeability
ras Proteins
Lentivirus
Protein Transport
Green Fluorescent Proteins
Small Interfering RNA
Microscopy
Microscopic examination
Chemical activation
Proteins

Keywords

  • Beta-adrenergic receptors
  • Lipopolysaccharide
  • Permeability
  • Rab1 GTPase
  • Small interfering RNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Rab1 GTPase promotes expression of β-adrenergic receptors in rat pulmonary microvascular endothelial cells. / Li, Yuncheng; Wang, Guansong; Lin, Kexiong; Yin, Hongjin; Zhou, Changxi; Liu, Ting; Wu, Guangyu; Qian, Guisheng.

In: International Journal of Biochemistry and Cell Biology, Vol. 42, No. 7, 01.07.2010, p. 1201-1209.

Research output: Contribution to journalArticle

Li, Yuncheng ; Wang, Guansong ; Lin, Kexiong ; Yin, Hongjin ; Zhou, Changxi ; Liu, Ting ; Wu, Guangyu ; Qian, Guisheng. / Rab1 GTPase promotes expression of β-adrenergic receptors in rat pulmonary microvascular endothelial cells. In: International Journal of Biochemistry and Cell Biology. 2010 ; Vol. 42, No. 7. pp. 1201-1209.
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AU - Zhou, Changxi

AU - Liu, Ting

AU - Wu, Guangyu

AU - Qian, Guisheng

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