Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice

Xiaoping Ao, Lujun Zhao, Mary A. Davis, David M. Lubman, Theodore S. Lawrence, Feng Ming Kong

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Background: Recent research has supported that a variety of cytokines play important roles during radiation-induced lung toxicity. The present study is designed to investigate the differences in early cytokine induction after radiation in sensitive (C57BL/6) and resistant mice (C3H). Results: Twenty-two cytokines in the lung tissue homogenates, bronchial lavage (BAL) fluids, and serum from 3, 6, 12, 24 hrs to 1 week after 12 Gy whole lung irradiation were profiled using a microsphere-based multiplexed cytokine assay. The majority of cytokines had similar baseline levels in C57BL/6 and C3H mice, but differed significantly after radiation. Many, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and keratinocyte-derived chemokine (KC) were elevated significantly in specimens from both strains. They usually peaked at about 3-6 hrs in C57BL/6 and 6-12 hrs in C3H. At 6 hrs in lung tissue, G-CSF, IL-6, and KC increased 6, 8, and 11 fold in C57BL/6 mice, 4, 3, and 3 fold in the C3H mice, respectively. IL-6 was 10-fold higher at 6 hrs in the C57BL/6 BAL fluid than the C3H BAL fluid. MCP-1, IP-10, and IL-1α also showed some differences between strains in the lung tissue and/or serum. For the same cytokine and within the same strain of mice, there were significant linear correlations between lung tissue and BAL fluid levels (R2 ranged 0.46-0.99) and between serum and tissue (R2 ranged 0.56-0.98). Conclusion: Radiation induced earlier and greater temporal changes in multiple cytokines in the pulmonary fibrosis sensitive mice. Positive correlation between serum and tissue levels suggests that blood may be used as a surrogate marker for tissue.

Original languageEnglish (US)
Article number6
JournalJournal of Hematology and Oncology
Volume2
DOIs
StatePublished - Sep 7 2009

Fingerprint

Radiation Pneumonitis
Bronchoalveolar Lavage Fluid
Radiation
Cytokines
Lung
Inbred C3H Mouse
Interleukin-6
Granulocyte Colony-Stimulating Factor
Serum
Inbred C57BL Mouse
Pulmonary Fibrosis
Interleukin-1
Microspheres
Biomarkers
Research

ASJC Scopus subject areas

  • Hematology
  • Molecular Biology
  • Oncology
  • Cancer Research

Cite this

Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice. / Ao, Xiaoping; Zhao, Lujun; Davis, Mary A.; Lubman, David M.; Lawrence, Theodore S.; Kong, Feng Ming.

In: Journal of Hematology and Oncology, Vol. 2, 6, 07.09.2009.

Research output: Contribution to journalArticle

Ao, Xiaoping ; Zhao, Lujun ; Davis, Mary A. ; Lubman, David M. ; Lawrence, Theodore S. ; Kong, Feng Ming. / Radiation produces differential changes in cytokine profiles in radiation lung fibrosis sensitive and resistant mice. In: Journal of Hematology and Oncology. 2009 ; Vol. 2.
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abstract = "Background: Recent research has supported that a variety of cytokines play important roles during radiation-induced lung toxicity. The present study is designed to investigate the differences in early cytokine induction after radiation in sensitive (C57BL/6) and resistant mice (C3H). Results: Twenty-two cytokines in the lung tissue homogenates, bronchial lavage (BAL) fluids, and serum from 3, 6, 12, 24 hrs to 1 week after 12 Gy whole lung irradiation were profiled using a microsphere-based multiplexed cytokine assay. The majority of cytokines had similar baseline levels in C57BL/6 and C3H mice, but differed significantly after radiation. Many, including granulocyte colony-stimulating factor (G-CSF), interleukin-6 (IL-6), and keratinocyte-derived chemokine (KC) were elevated significantly in specimens from both strains. They usually peaked at about 3-6 hrs in C57BL/6 and 6-12 hrs in C3H. At 6 hrs in lung tissue, G-CSF, IL-6, and KC increased 6, 8, and 11 fold in C57BL/6 mice, 4, 3, and 3 fold in the C3H mice, respectively. IL-6 was 10-fold higher at 6 hrs in the C57BL/6 BAL fluid than the C3H BAL fluid. MCP-1, IP-10, and IL-1α also showed some differences between strains in the lung tissue and/or serum. For the same cytokine and within the same strain of mice, there were significant linear correlations between lung tissue and BAL fluid levels (R2 ranged 0.46-0.99) and between serum and tissue (R2 ranged 0.56-0.98). Conclusion: Radiation induced earlier and greater temporal changes in multiple cytokines in the pulmonary fibrosis sensitive mice. Positive correlation between serum and tissue levels suggests that blood may be used as a surrogate marker for tissue.",
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