Rana catesbeiana virus Z (RCV-Z): A novel pathogenic ranavirus

Sai Majji, Scott LaPatra, Scott M. Long, Robert Sample, Locke Bryan, Allan Sinning, V. Gregory Chinchar

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A virus, designated Rana catesbeiana virus Z (RCV-Z), was isolated from the visceral tissue of moribund tadpoles of the North American bullfrog Rana catesbeiana. SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) analysis of viral proteins and sequence analysis of the amino terminal end of the major capsid protein showed that RCV-Z was similar to frog virus 3 (FV3) and other ranaviruses isolated from anurans and fish. However, analysis of restriction fragment profiles following digestion of viral genomic DNA with XbaI and BamHI indicated that RCV-Z was markedly different from FV3. Moreover, in contrast to FV3, RCV-Z contained a full-length copy of the viral homolog of eukaryotic initiation factor 2 alpha (eIF-2α). Experimental infection of bullfrog tadpoles with FV3 and RCV-Z demonstrated that RCV-Z was much more pathogenic than FV3, and that prior infection with FV3 protected them from subsequent RCV-Z induced mortality. Collectively, these results suggest that RCV-Z may represent a novel species of ranavirus capable of infecting frogs and that possession of a viral eIF-2α homolog (vIF-2α) correlates with enhanced virulence.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalDiseases of Aquatic Organisms
Volume73
Issue number1
DOIs
StatePublished - Nov 21 2006

Fingerprint

Ranavirus
Lithobates catesbeianus
Frog virus 3
virus
viruses
frog
tadpoles
viral proteins
coat proteins
infection
polyacrylamide gel electrophoresis
frogs
protein
virulence
sequence analysis
digestion

Keywords

  • Frog virus 3
  • Iridovirus
  • Rana catesbeiana virus Z
  • Ranavirus
  • Virulence
  • eiF-2α homolog

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Majji, S., LaPatra, S., Long, S. M., Sample, R., Bryan, L., Sinning, A., & Chinchar, V. G. (2006). Rana catesbeiana virus Z (RCV-Z): A novel pathogenic ranavirus. Diseases of Aquatic Organisms, 73(1), 1-11. https://doi.org/10.3354/dao073001

Rana catesbeiana virus Z (RCV-Z) : A novel pathogenic ranavirus. / Majji, Sai; LaPatra, Scott; Long, Scott M.; Sample, Robert; Bryan, Locke; Sinning, Allan; Chinchar, V. Gregory.

In: Diseases of Aquatic Organisms, Vol. 73, No. 1, 21.11.2006, p. 1-11.

Research output: Contribution to journalArticle

Majji, S, LaPatra, S, Long, SM, Sample, R, Bryan, L, Sinning, A & Chinchar, VG 2006, 'Rana catesbeiana virus Z (RCV-Z): A novel pathogenic ranavirus', Diseases of Aquatic Organisms, vol. 73, no. 1, pp. 1-11. https://doi.org/10.3354/dao073001
Majji, Sai ; LaPatra, Scott ; Long, Scott M. ; Sample, Robert ; Bryan, Locke ; Sinning, Allan ; Chinchar, V. Gregory. / Rana catesbeiana virus Z (RCV-Z) : A novel pathogenic ranavirus. In: Diseases of Aquatic Organisms. 2006 ; Vol. 73, No. 1. pp. 1-11.
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