Rapid decrease of RNA level of a novel mouse mitochondria solute carrier protein (Mscp) gene at 4-5 weeks of age

Quan Zhen Li, Sarah Eckenrode, Qing Guo Ruan, Cong Yi Wang, Jing Da Shi, Richard A McIndoe, Jin-Xiong She

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We cloned a novel mouse gene that encodes a protein with homology to the mitochondria solute carrier proteins (Mscp). The major full-length Mscp transcript contains 4112 bp of cDNA and a deduced protein of 338 amino acids. The Mscp protein shares 50%, 40%, and 39% sequence identity with the C. elegans hypothetical protein T26089 and the yeast mitochondria carrier proteins MRS3 and MRS4, respectively. It also showed homology with the uncoupling proteins (UCP1, UCP2, and UCP3; 22%, 24%, and 29% identity, respectively). The protein has six trans-membrane domains and three mitochondria energy-transfer protein signature motifs, which are conserved among all the members of mitochondria carrier protein family. Northern analysis indicated that the Mscp gene is highly expressed in the spleen. Using cDNA microarray and Northern analysis, we have shown a significant decrease of the splenic Mscp mRNA levels around 4-5 weeks of age in several mouse strains including C57BL/6J, nonobese diabetic (NOD), and several NOD-congenic mice. These results suggest that the Mscp gene is decreased during splenic lymphocyte maturation in these mice.

Original languageEnglish (US)
Pages (from-to)830-836
Number of pages7
JournalMammalian Genome
Volume12
Issue number11
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Mitochondria
RNA
Genes
Carrier Proteins
Proteins
Caenorhabditis elegans Proteins
Congenic Mice
Mouse Mscp protein
Inbred NOD Mouse
Amino Acid Motifs
Energy Transfer
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Solute Carrier Proteins
Spleen
Complementary DNA
Yeasts
Lymphocytes
Amino Acids
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Rapid decrease of RNA level of a novel mouse mitochondria solute carrier protein (Mscp) gene at 4-5 weeks of age. / Li, Quan Zhen; Eckenrode, Sarah; Ruan, Qing Guo; Wang, Cong Yi; Shi, Jing Da; McIndoe, Richard A; She, Jin-Xiong.

In: Mammalian Genome, Vol. 12, No. 11, 01.01.2001, p. 830-836.

Research output: Contribution to journalArticle

Li, Quan Zhen ; Eckenrode, Sarah ; Ruan, Qing Guo ; Wang, Cong Yi ; Shi, Jing Da ; McIndoe, Richard A ; She, Jin-Xiong. / Rapid decrease of RNA level of a novel mouse mitochondria solute carrier protein (Mscp) gene at 4-5 weeks of age. In: Mammalian Genome. 2001 ; Vol. 12, No. 11. pp. 830-836.
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