Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide

Ayman Al-Hendy, Paavo Toivanen, Mikael Skurnik

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem™ both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

Original languageEnglish (US)
Pages (from-to)741-753
Number of pages13
JournalMicrobiology and Immunology
Volume35
Issue number4
DOIs
StatePublished - Jan 1 1991

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Lipopolysaccharides
Staining and Labeling
Silver Staining
Phenol
Gels
Water

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide. / Al-Hendy, Ayman; Toivanen, Paavo; Skurnik, Mikael.

In: Microbiology and Immunology, Vol. 35, No. 4, 01.01.1991, p. 741-753.

Research output: Contribution to journalArticle

Al-Hendy, Ayman ; Toivanen, Paavo ; Skurnik, Mikael. / Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide. In: Microbiology and Immunology. 1991 ; Vol. 35, No. 4. pp. 741-753.
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