Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide

Ayman Al-Hendy, Paavo Toivanen, Mikael Skurnik

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem™ both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol-water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.

Original languageEnglish (US)
Pages (from-to)741-753
Number of pages13
JournalMicrobiology and Immunology
Volume35
Issue number4
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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