Ratiometric quantitation of thiol metabolites using non-isotopic mass tags

Xiaofeng Zhao, Dawn S. Hui, Richard Lee, James L. Edwards

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Ratiometric quantitation is used in mass spectrometry to account for variations in ionization efficiencies due to heterogenous sample matrixes. Isotopes are most commonly used to achieve ratiometric quantitation because of their ability to co-elute chromatographically with each other and to have similar ionization efficiencies. In the work presented here, a new non-isotopic quantitative tagging approach is presented which allows chromatographic co-elution and similar ionization efficiencies. Using two variations of maleimide tags, t-butyl and cyclohexyl maleimide, thiols are quantified with a high degree of linearity up to five-fold concentration differences. Because these two tags have similar hydrophobcities, they elute simultaneously which allows them to be used for ratiometric quantitation. Beyond the five-fold linear range, signal compression is observed. This technique was able to quantify thiol changes in both in vitro pharmacological treatments as well as in vivo diabetic tissue.

Original languageEnglish (US)
Pages (from-to)274-280
Number of pages7
JournalAnalytica Chimica Acta
Volume1037
DOIs
StatePublished - Dec 11 2018
Externally publishedYes

Fingerprint

thiol
Metabolites
Sulfhydryl Compounds
Ionization
metabolite
ionization
Isotopes
fold
Mass Spectrometry
tagging
Pharmacology
linearity
Mass spectrometry
mass spectrometry
compression
isotope
Tissue
matrix
maleimide
In Vitro Techniques

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Ratiometric quantitation of thiol metabolites using non-isotopic mass tags. / Zhao, Xiaofeng; Hui, Dawn S.; Lee, Richard; Edwards, James L.

In: Analytica Chimica Acta, Vol. 1037, 11.12.2018, p. 274-280.

Research output: Contribution to journalArticle

Zhao, Xiaofeng ; Hui, Dawn S. ; Lee, Richard ; Edwards, James L. / Ratiometric quantitation of thiol metabolites using non-isotopic mass tags. In: Analytica Chimica Acta. 2018 ; Vol. 1037. pp. 274-280.
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