Rational design and theoretical optimization of a cryopreservation protocol

Jens O.M. Karlsson, Ali Eroglu, Thomas L. Toth, Ernest G. Cravalho, Mehmet Toner

Research output: Contribution to journalConference article

6 Citations (Scopus)

Abstract

A cryopreservation protocol for mouse oocytes was developed by rational design and theoretical optimization using a physicochemical model of the intracellular ice formation process. Theoretical models of freeze-induced cell dehydration, homogeneous and heterogeneous nucleation of intracellular ice, and diffusion-limited crystal growth of intracellular ice were coupled and solved numerically to obtain predictions of cell response to freezing. A two-step, piecewise linear cooling protocol was chosen based on simulation results, and protocol parameters were then optimized using the mathematical model. The predicted optimal parameters were an initial cooling rate of Bdehydration = 0.59 °C/min and a plunge temperature of Tplunge = -67 °C. Experimental testing showed that optimal survival (78% cells morphologically normal) was obtained at Bdehydration = 0.5 °C/min and Tplunge = -80 °C. The fertilization rate and blastocyst formation rate of oocytes cryopreserved using the optimized protocol were 65% and 49%, respectively.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalAmerican Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD
Volume322
StatePublished - Dec 1 1995
EventProceedings of the 1995 ASME International Mechanical Engineering Congress and Exposition - San Francisco, CA, USA
Duration: Nov 12 1995Nov 17 1995

Fingerprint

Ice
Cooling
Crystallization
Dehydration
Crystal growth
Freezing
Nucleation
Mathematical models
Testing
Temperature

ASJC Scopus subject areas

  • Mechanical Engineering
  • Fluid Flow and Transfer Processes

Cite this

Rational design and theoretical optimization of a cryopreservation protocol. / Karlsson, Jens O.M.; Eroglu, Ali; Toth, Thomas L.; Cravalho, Ernest G.; Toner, Mehmet.

In: American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD, Vol. 322, 01.12.1995, p. 85-89.

Research output: Contribution to journalConference article

@article{a71576dbbed945c89fc6ed8d158760c1,
title = "Rational design and theoretical optimization of a cryopreservation protocol",
abstract = "A cryopreservation protocol for mouse oocytes was developed by rational design and theoretical optimization using a physicochemical model of the intracellular ice formation process. Theoretical models of freeze-induced cell dehydration, homogeneous and heterogeneous nucleation of intracellular ice, and diffusion-limited crystal growth of intracellular ice were coupled and solved numerically to obtain predictions of cell response to freezing. A two-step, piecewise linear cooling protocol was chosen based on simulation results, and protocol parameters were then optimized using the mathematical model. The predicted optimal parameters were an initial cooling rate of Bdehydration = 0.59 °C/min and a plunge temperature of Tplunge = -67 °C. Experimental testing showed that optimal survival (78{\%} cells morphologically normal) was obtained at Bdehydration = 0.5 °C/min and Tplunge = -80 °C. The fertilization rate and blastocyst formation rate of oocytes cryopreserved using the optimized protocol were 65{\%} and 49{\%}, respectively.",
author = "Karlsson, {Jens O.M.} and Ali Eroglu and Toth, {Thomas L.} and Cravalho, {Ernest G.} and Mehmet Toner",
year = "1995",
month = "12",
day = "1",
language = "English (US)",
volume = "322",
pages = "85--89",
journal = "American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD",
issn = "0272-5673",
publisher = "American Society of Mechanical Engineers(ASME)",

}

TY - JOUR

T1 - Rational design and theoretical optimization of a cryopreservation protocol

AU - Karlsson, Jens O.M.

AU - Eroglu, Ali

AU - Toth, Thomas L.

AU - Cravalho, Ernest G.

AU - Toner, Mehmet

PY - 1995/12/1

Y1 - 1995/12/1

N2 - A cryopreservation protocol for mouse oocytes was developed by rational design and theoretical optimization using a physicochemical model of the intracellular ice formation process. Theoretical models of freeze-induced cell dehydration, homogeneous and heterogeneous nucleation of intracellular ice, and diffusion-limited crystal growth of intracellular ice were coupled and solved numerically to obtain predictions of cell response to freezing. A two-step, piecewise linear cooling protocol was chosen based on simulation results, and protocol parameters were then optimized using the mathematical model. The predicted optimal parameters were an initial cooling rate of Bdehydration = 0.59 °C/min and a plunge temperature of Tplunge = -67 °C. Experimental testing showed that optimal survival (78% cells morphologically normal) was obtained at Bdehydration = 0.5 °C/min and Tplunge = -80 °C. The fertilization rate and blastocyst formation rate of oocytes cryopreserved using the optimized protocol were 65% and 49%, respectively.

AB - A cryopreservation protocol for mouse oocytes was developed by rational design and theoretical optimization using a physicochemical model of the intracellular ice formation process. Theoretical models of freeze-induced cell dehydration, homogeneous and heterogeneous nucleation of intracellular ice, and diffusion-limited crystal growth of intracellular ice were coupled and solved numerically to obtain predictions of cell response to freezing. A two-step, piecewise linear cooling protocol was chosen based on simulation results, and protocol parameters were then optimized using the mathematical model. The predicted optimal parameters were an initial cooling rate of Bdehydration = 0.59 °C/min and a plunge temperature of Tplunge = -67 °C. Experimental testing showed that optimal survival (78% cells morphologically normal) was obtained at Bdehydration = 0.5 °C/min and Tplunge = -80 °C. The fertilization rate and blastocyst formation rate of oocytes cryopreserved using the optimized protocol were 65% and 49%, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0029423477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029423477&partnerID=8YFLogxK

M3 - Conference article

AN - SCOPUS:0029423477

VL - 322

SP - 85

EP - 89

JO - American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD

JF - American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD

SN - 0272-5673

ER -