Reaction of hemoglobin with HOCl

Mechanism of heme destruction and free iron release

Dhiman Maitra, Jaeman Byun, Peter R. Andreana, Ibrahim Abdulhamid, Michael Peter Diamond, Ghassan M. Saed, Subramaniam Pennathur, Husam M. Abu-Soud

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Hypochlorous acid (HOCl) is generated by myeloperoxidase using chloride and hydrogen peroxide as substrates. HOCl and its conjugate base (OCl-) bind to the heme moiety of hemoglobin (Hb) and generate a transient ferric species whose formation and decay kinetics indicate it can participate in protein aggregation and heme destruction along with subsequent free iron release. The oxidation of the Hb heme moiety by OCl- was accompanied by marked heme destruction as judged by the decrease in and subsequent flattening of the Soret absorbance peak at 405 nm. HOCl-mediated Hb heme depletion was confirmed by HPLC analysis and in-gel heme staining. Exposure of Hb to increasing concentrations of HOCl produced a number of porphyrin degradation products resulting from oxidative cleavage of one or more of the carbon-methene bridges of the tetrapyrrole ring, as identified by their characteristic HPLC fluorescence and LC-MS. A nonreducing denaturing SDS-PAGE showed several degrees of protein aggregation. Similarly, porphyrin degradation products were identified after exposure of red blood cells to increasing concentrations of HOCl, indicating biological relevance of this finding. This work provides a direct link between Hb heme destruction and subsequent free iron accumulation, as occurs under inflammatory conditions where HOCl is formed in substantial amounts.

Original languageEnglish (US)
Pages (from-to)374-386
Number of pages13
JournalFree Radical Biology and Medicine
Volume51
Issue number2
DOIs
StatePublished - Jul 15 2011
Externally publishedYes

Fingerprint

Hypochlorous Acid
Heme
Hemoglobins
Iron
Porphyrins
Agglomeration
High Pressure Liquid Chromatography
Tetrapyrroles
Degradation
Hydrogen Peroxide
Peroxidase
Chlorides
Polyacrylamide Gel Electrophoresis
Proteins
Blood
Carbon
Erythrocytes
Fluorescence
Gels
Cells

Keywords

  • Free iron
  • Free radicals
  • Hemoglobin
  • Hypochlorous acid
  • Inflammation
  • Mammalian peroxidase
  • Oxidative stress
  • RBC

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Reaction of hemoglobin with HOCl : Mechanism of heme destruction and free iron release. / Maitra, Dhiman; Byun, Jaeman; Andreana, Peter R.; Abdulhamid, Ibrahim; Diamond, Michael Peter; Saed, Ghassan M.; Pennathur, Subramaniam; Abu-Soud, Husam M.

In: Free Radical Biology and Medicine, Vol. 51, No. 2, 15.07.2011, p. 374-386.

Research output: Contribution to journalArticle

Maitra, D, Byun, J, Andreana, PR, Abdulhamid, I, Diamond, MP, Saed, GM, Pennathur, S & Abu-Soud, HM 2011, 'Reaction of hemoglobin with HOCl: Mechanism of heme destruction and free iron release', Free Radical Biology and Medicine, vol. 51, no. 2, pp. 374-386. https://doi.org/10.1016/j.freeradbiomed.2011.04.011
Maitra, Dhiman ; Byun, Jaeman ; Andreana, Peter R. ; Abdulhamid, Ibrahim ; Diamond, Michael Peter ; Saed, Ghassan M. ; Pennathur, Subramaniam ; Abu-Soud, Husam M. / Reaction of hemoglobin with HOCl : Mechanism of heme destruction and free iron release. In: Free Radical Biology and Medicine. 2011 ; Vol. 51, No. 2. pp. 374-386.
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