Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation

Hassan Sellak, Elisabeth Franzini, Jacques Hakim, Catherine Pasquier

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

We compared the effects of phorbol 12-myristate 13-acetate (PMA) and thrombin with those of nonlytic concentrations of reactive oxygen species (ROS) generated by hypoxanthine (HX)-xanthine oxidase (XO) on the adhesion properties of human umbilical cord vein endothelial cells (HUVEC) to resting polymorphonuclear neutrophils (PMN). PMN adherence to HX-XO-treated HUVEC was increased approximately twofold to 2.5-fold relative to untreated HUVEC, both immediately and after 2 hours. It was not additive to that induced by PMA or thrombin stimulation of HUVEC. ROS-induced adherence was not due to platelet- activating factor (PAF) or P-selectin expression, as it was neither antagonized by BN52021 (PAF receptor antagonist) nor inhibited by anti-P- selectin monoclonal antibody (MoAb), contrary to the increased adhesion of PMA- and thrombin-stimulated HUVEC. PMN preincubated with mannose-6-P or N- acetylneuraminic acid (sialic acid), but not mannose or galactose-6-P, showed reduced adherence to ROS-treated HUVEC, suggesting that carbohydrate molecules were expressed on the latter and served as the ligand for the PMN L-selectin. Intercellular adhesion molecule (ICAM-1), constitutively present on the surface of resting HUVEC, was involved in the PMN adherence to ROS- treated HUVEC, since this adherence was inhibited by anti-ICAM-1, anti- CD11a, anti-CD11b, and anti-CD18 MoAbs. An non-CD18, non-ICAM-1-dependent mechanism is also involved in this adherence, since effects of these MoAbs were not additive; moreover, combinations of anti-CD18 and anti-ICAM-1 MoAbs with mannose-6-P and sialic acid completely inhibited PMN adherence. The increased binding of PMN to HX-XO-exposed HUVEC observed here involved ICAM- 1, but was independent of its upregulation, and another non-ICAM-1-dependent mechanism, in which carbohydrates expressed on HUVEC recognize L-selectin on PMN.

Original languageEnglish (US)
Pages (from-to)2669-2677
Number of pages9
JournalBlood
Volume83
Issue number9
StatePublished - May 1 1994

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Umbilical Cord
Endothelial cells
Human Umbilical Vein Endothelial Cells
Intercellular Adhesion Molecule-1
Reactive Oxygen Species
Neutrophils
Up-Regulation
Xanthine Oxidase
N-Acetylneuraminic Acid
Mannose
Thrombin
L-Selectin
Acetates
P-Selectin
Adhesion
Carbohydrates
Platelet Activating Factor
Cell Adhesion Molecules
Galactose
Monoclonal Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation. / Sellak, Hassan; Franzini, Elisabeth; Hakim, Jacques; Pasquier, Catherine.

In: Blood, Vol. 83, No. 9, 01.05.1994, p. 2669-2677.

Research output: Contribution to journalArticle

Sellak, H, Franzini, E, Hakim, J & Pasquier, C 1994, 'Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation', Blood, vol. 83, no. 9, pp. 2669-2677.
Sellak, Hassan ; Franzini, Elisabeth ; Hakim, Jacques ; Pasquier, Catherine. / Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation. In: Blood. 1994 ; Vol. 83, No. 9. pp. 2669-2677.
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