Recombinant human collagen for tissue engineered corneal substitutes

Wenguang Liu, Kimberley Merrett, May Griffith, Per Fagerholm, Subhadra Dravida, Belinda Heyne, Juan C. Scaiano, Mitchell A. Watsky, Naoshi Shinozaki, Neil Lagali, Rejean Munger, Fengfu Li

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

We successfully fabricated transparent, robust hydrogels as corneal substitutes from concentrated recombinant human type I and type III collagen solutions crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). White light transmission through these gels is comparable or superior to that of human corneas. Hydrogels from both type I and type III collagens supported in vitro epithelium and nerve over-growth. While both these biocompatible hydrogels have adequate tensile strength and elasticity for surgical manipulation, type III collagen hydrogels tended to be mechanically superior. Twelve-month post-implantation results of type I recombinant collagen-based corneal substitutes into mini-pigs showed retention of optical clarity, along with regeneration of corneal cells, nerves and tear film. For clinical use, implants based on fully characterized, recombinant human collagen eliminate the risk of pathogen transfer or xenogeneic immuno-responses posed by animal collagens.

Original languageEnglish (US)
Pages (from-to)1147-1158
Number of pages12
JournalBiomaterials
Volume29
Issue number9
DOIs
StatePublished - Mar 1 2008

Fingerprint

Hydrogels
Collagen
Collagen Type III
Collagen Type I
Tissue
Tensile Strength
Elasticity
Pathogens
Light transmission
Tears
Cornea
Neurons
Regeneration
Animals
Tensile strength
Swine
Epithelium
Gels
Light
Growth

Keywords

  • Cornea regeneration
  • Cornea transplantation
  • Corneal substitute
  • Hydrogel
  • Recombinant human collagen

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Liu, W., Merrett, K., Griffith, M., Fagerholm, P., Dravida, S., Heyne, B., ... Li, F. (2008). Recombinant human collagen for tissue engineered corneal substitutes. Biomaterials, 29(9), 1147-1158. https://doi.org/10.1016/j.biomaterials.2007.11.011

Recombinant human collagen for tissue engineered corneal substitutes. / Liu, Wenguang; Merrett, Kimberley; Griffith, May; Fagerholm, Per; Dravida, Subhadra; Heyne, Belinda; Scaiano, Juan C.; Watsky, Mitchell A.; Shinozaki, Naoshi; Lagali, Neil; Munger, Rejean; Li, Fengfu.

In: Biomaterials, Vol. 29, No. 9, 01.03.2008, p. 1147-1158.

Research output: Contribution to journalArticle

Liu, W, Merrett, K, Griffith, M, Fagerholm, P, Dravida, S, Heyne, B, Scaiano, JC, Watsky, MA, Shinozaki, N, Lagali, N, Munger, R & Li, F 2008, 'Recombinant human collagen for tissue engineered corneal substitutes', Biomaterials, vol. 29, no. 9, pp. 1147-1158. https://doi.org/10.1016/j.biomaterials.2007.11.011
Liu W, Merrett K, Griffith M, Fagerholm P, Dravida S, Heyne B et al. Recombinant human collagen for tissue engineered corneal substitutes. Biomaterials. 2008 Mar 1;29(9):1147-1158. https://doi.org/10.1016/j.biomaterials.2007.11.011
Liu, Wenguang ; Merrett, Kimberley ; Griffith, May ; Fagerholm, Per ; Dravida, Subhadra ; Heyne, Belinda ; Scaiano, Juan C. ; Watsky, Mitchell A. ; Shinozaki, Naoshi ; Lagali, Neil ; Munger, Rejean ; Li, Fengfu. / Recombinant human collagen for tissue engineered corneal substitutes. In: Biomaterials. 2008 ; Vol. 29, No. 9. pp. 1147-1158.
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