Recombinant Tumor Necrosis Factor-α Chronically Administered in Rats

Lack of Cachectic Effect

Barbara J. Mullen, Ruth Babette Harris, John S. Patton, Roy J. Martin

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Recombinant human tumor necrosis factor-α (rHuTNF) was injected into rats to test its reported cachectic effects. Rats were subcutaneously injected daily at 1730 hr with either saline or rHuTNF (0.25 mg/kg body wt) for either 5 or 14 days. Daily food intakes were significantly depressed only for the first day and first two days of rHuTNF injection in animals treated for 5 days and 14 days, respectively. There were no significant differences in daily body weights among the groups. Analysis of carcass composition revealed no significant differences in percentage of lipid or protein. Liver and inguinal pad weights were not significantly different. In vitro determination of lipogenesis showed it was enhanced in the inguinal pad and depressed in the liver only after 14 days of treatment. These results demonstrate that although in vivo rHuTNF may specifically alter tissue metabolism, it does not, by itself, result in a sustained cachectic effect.

Original languageEnglish (US)
Pages (from-to)318-325
Number of pages8
JournalProceedings of the Society for Experimental Biology and Medicine
Volume193
Issue number4
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

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Rats
Tumor Necrosis Factor-alpha
Groin
Liver
Lipogenesis
Metabolism
Animals
Eating
Body Weight
Tissue
Lipids
Weights and Measures
Injections
human TNF protein
Chemical analysis
Proteins
Therapeutics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Recombinant Tumor Necrosis Factor-α Chronically Administered in Rats : Lack of Cachectic Effect. / Mullen, Barbara J.; Harris, Ruth Babette; Patton, John S.; Martin, Roy J.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 193, No. 4, 01.01.1990, p. 318-325.

Research output: Contribution to journalArticle

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