Recurrent spontaneous spreading depolarizations facilitate acute dendritic injury in the ischemic penumbra

W. Christopher Risher, Deborah Ard, Jianghe Yuan, Sergei A Kirov

Research output: Contribution to journalArticle

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Abstract

Spontaneous spreading depolarizations (SDs) occur in the penumbra surrounding ischemic core. These SDs, often referred to as peri-infarct depolarizations, cause vasoconstriction and recruitment of the penumbra into the ischemic core in the critical first hours after focal ischemic stroke; however, the real-time spatiotemporal dynamics of SD-induced injury to synaptic circuitry in the penumbra remain unknown. A modified cortical photothrombosis model was used to produce a square-shaped lesion surrounding a penumbra-like "area at risk" in middle cerebral artery territory of mouse somatosensory cortex. Lesioning resulted in recurrent spontaneous SDs. In vivo two-photon microscopy of green fluorescent protein-expressing neurons in this penumbra-like area at risk revealed that SDs were temporally correlated with rapid (<6 s) dendritic beading. Dendrites quickly (<3 min) recovered between SDs to near-control morphology until the occurrence of SD-induced terminal dendritic injury, signifying acute synaptic damage. SDs are characterized by a break-down of ion homeostasis that can be recovered by ion pumps if the energy supply is adequate. Indeed, the likelihood of rapid dendritic recovery between SDs was correlated with the presence of nearby flowing blood vessels, but the presence of such vessels was not always sufficient for rapid dendritic recovery, suggesting that energy needs for recovery exceeded energy supply of compromised blood flow. We propose that metabolic stress resulting from recurring SDs facilitates acute injury at the level of dendrites and dendritic spines in metabolically compromised tissue, expediting penumbral recruitment into the ischemic core.

Original languageEnglish (US)
Pages (from-to)9859-9868
Number of pages10
JournalJournal of Neuroscience
Volume30
Issue number29
DOIs
StatePublished - Jul 21 2010

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Dendrites
Wounds and Injuries
Ion Pumps
Dendritic Spines
Physiological Stress
Somatosensory Cortex
Middle Cerebral Artery
Green Fluorescent Proteins
Vasoconstriction
Photons
Blood Vessels
Microscopy
Homeostasis
Stroke
Ions
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Recurrent spontaneous spreading depolarizations facilitate acute dendritic injury in the ischemic penumbra. / Risher, W. Christopher; Ard, Deborah; Yuan, Jianghe; Kirov, Sergei A.

In: Journal of Neuroscience, Vol. 30, No. 29, 21.07.2010, p. 9859-9868.

Research output: Contribution to journalArticle

Risher, W. Christopher ; Ard, Deborah ; Yuan, Jianghe ; Kirov, Sergei A. / Recurrent spontaneous spreading depolarizations facilitate acute dendritic injury in the ischemic penumbra. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 29. pp. 9859-9868.
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