Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin

P. Suppa, A. Ruggeri, F. R. Toy, C. Prati, M. Biasotto, M. Falconi, David Henry Pashley, L. Breschi

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and normal hard dentin. A double-immunolabeling technique was performed on these sections, with anti-type I collagen and anti-chondroitin 4/6 sulfate monoclonal primary antibodies. We used gold-conjugated secondary antibodies to visualize the distribution of intact collagen fibrils and proteoglycans by high-resolution SEM. For sclerotic dentin, labeling densities were 19.57 ± 3.01/μm2 for collagen and 9.84 ± 2.62/μm2 for proteoglycans. For normal hard dentin, values were 35.20 ± 2.73/μm2 and 17.03 ± 1.98/μm2, respectively. Distribution of intact collagen fibrils and proteoglycans in sclerotic dentin was significantly lower than in normal hard dentin. Reductions in antigenicity from the organic matrix of sclerotic dentin under caries lesions raise concern about the potential of intrafibrillar remineralization.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalJournal of Dental Research
Volume85
Issue number2
DOIs
StatePublished - Feb 1 2006

Keywords

  • Caries
  • Collagen fibrils
  • FEISEM
  • Immunohistochemistry
  • Proteoglycans

ASJC Scopus subject areas

  • Dentistry(all)

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    Suppa, P., Ruggeri, A., Toy, F. R., Prati, C., Biasotto, M., Falconi, M., Pashley, D. H., & Breschi, L. (2006). Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Journal of Dental Research, 85(2), 133-137. https://doi.org/10.1177/154405910608500204