Reduced efficacy of multiple doses of CpG-matured dendritic cell tumor vaccine in an experimental model

Arash Pourgholaminejad, Arezoo Jamali, Morteza Samadi-Foroushani, Afshin Amari, Reza Mirzaei, Bita Ansaripour, Nemat Khansari, Mohammad Reza Aghasadeghi, Babak Baban, Jamshid Hadjati

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

CpG motifs have been advanced as agents that stimulate the maturation of DCs for immunotherapy. The present study tested the hypothesis that multiple doses of CpG-matured DC vaccine would be efficacious for complete eradication of experimentally-induced tumor. Accordingly, WEHI164 cells were implanted subcutaneously in the flank of BALB/c mice. During DC culture, tumor lysate was added to immature DCs followed by addition of CpG or non-CpG control 4-6 h later. A total of three doses of CpG or non-CpG control-matured DCs were injected around tumors. The results showed that multiple doses of CpG-matured DCs led to considerable decrease in cytotoxicity of lymphocytes and significantly increased tumor growth rate compared to a single dose. Further, mice which received three doses of the vaccine also displayed significant FoxP3 in tumor tissue. In conclusion, multiple doses of CpG-matured DCs exhibited decreased anti-tumor immunity in association with increased expression of FoxP3.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalCellular Immunology
Volume271
Issue number2
DOIs
StatePublished - Sep 1 2011

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Cancer Vaccines
Dendritic Cells
Theoretical Models
Neoplasms
Vaccines
Immunotherapy
Immunity
Lymphocytes
Growth

Keywords

  • Cell therapy
  • FoxP3
  • Synthetic oligodeoxynucleotides
  • Tumor immunotherapy

ASJC Scopus subject areas

  • Immunology

Cite this

Pourgholaminejad, A., Jamali, A., Samadi-Foroushani, M., Amari, A., Mirzaei, R., Ansaripour, B., ... Hadjati, J. (2011). Reduced efficacy of multiple doses of CpG-matured dendritic cell tumor vaccine in an experimental model. Cellular Immunology, 271(2), 360-364. https://doi.org/10.1016/j.cellimm.2011.08.001

Reduced efficacy of multiple doses of CpG-matured dendritic cell tumor vaccine in an experimental model. / Pourgholaminejad, Arash; Jamali, Arezoo; Samadi-Foroushani, Morteza; Amari, Afshin; Mirzaei, Reza; Ansaripour, Bita; Khansari, Nemat; Aghasadeghi, Mohammad Reza; Baban, Babak; Hadjati, Jamshid.

In: Cellular Immunology, Vol. 271, No. 2, 01.09.2011, p. 360-364.

Research output: Contribution to journalArticle

Pourgholaminejad, A, Jamali, A, Samadi-Foroushani, M, Amari, A, Mirzaei, R, Ansaripour, B, Khansari, N, Aghasadeghi, MR, Baban, B & Hadjati, J 2011, 'Reduced efficacy of multiple doses of CpG-matured dendritic cell tumor vaccine in an experimental model', Cellular Immunology, vol. 271, no. 2, pp. 360-364. https://doi.org/10.1016/j.cellimm.2011.08.001
Pourgholaminejad A, Jamali A, Samadi-Foroushani M, Amari A, Mirzaei R, Ansaripour B et al. Reduced efficacy of multiple doses of CpG-matured dendritic cell tumor vaccine in an experimental model. Cellular Immunology. 2011 Sep 1;271(2):360-364. https://doi.org/10.1016/j.cellimm.2011.08.001
Pourgholaminejad, Arash ; Jamali, Arezoo ; Samadi-Foroushani, Morteza ; Amari, Afshin ; Mirzaei, Reza ; Ansaripour, Bita ; Khansari, Nemat ; Aghasadeghi, Mohammad Reza ; Baban, Babak ; Hadjati, Jamshid. / Reduced efficacy of multiple doses of CpG-matured dendritic cell tumor vaccine in an experimental model. In: Cellular Immunology. 2011 ; Vol. 271, No. 2. pp. 360-364.
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