Reduced motility longevity in thawed human spermatozoa

B. A. Keel, J. B. Black

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Fifty ejaculates from donors serving on an AID panel were divided into two 0.5 aliquots and evaluated for initial motility and kinetics. The first aliquot was then diluted with Tyrode solution and incubated in a Tyrode atmosphere at 37°C. Motility and kinetics were evaluated at 1, 3, 6, and 9 hr. The second aliquot was frozen and thawed 24 hr later. The aliquot was then diluted with Tyrode solution, incubated, and evaluated as before. The results indicate a significant reduction in motility longevity of the thawed aliquots. A significant reduction in kinetics of the thawed aliquots was also discovered. These findings suggest that decreased motility longevity of thawed semen may offer an explanation for reduced pregnancy rates using cryopreserved semen.

Original languageEnglish (US)
Pages (from-to)213-215
Number of pages3
JournalSystems Biology in Reproductive Medicine
Volume4
Issue number3
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

Keywords

  • Frozen Spermatozoa
  • Sperm
  • Sperm motility

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

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