Reduced motility longevity in thawed human spermatozoa

Brooks Allen Keel, J. B. Black

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Fifty ejaculates from donors serving on an AID panel were divided into two 0.5 aliquots and evaluated for initial motility and kinetics. The first aliquot was then diluted with Tyrode solution and incubated in a Tyrode atmosphere at 37°C. Motility and kinetics were evaluated at 1, 3, 6, and 9 hr. The second aliquot was frozen and thawed 24 hr later. The aliquot was then diluted with Tyrode solution, incubated, and evaluated as before. The results indicate a significant reduction in motility longevity of the thawed aliquots. A significant reduction in kinetics of the thawed aliquots was also discovered. These findings suggest that decreased motility longevity of thawed semen may offer an explanation for reduced pregnancy rates using cryopreserved semen.

Original languageEnglish (US)
Pages (from-to)213-215
Number of pages3
JournalSystems Biology in Reproductive Medicine
Volume4
Issue number3
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

Fingerprint

Spermatozoa
Semen
Pregnancy Rate
Atmosphere
Tyrode's solution

Keywords

  • Frozen Spermatozoa
  • Sperm
  • Sperm motility

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

Cite this

Reduced motility longevity in thawed human spermatozoa. / Keel, Brooks Allen; Black, J. B.

In: Systems Biology in Reproductive Medicine, Vol. 4, No. 3, 01.01.1980, p. 213-215.

Research output: Contribution to journalArticle

@article{e0a8d116b22b437893ef7ee05a7d5683,
title = "Reduced motility longevity in thawed human spermatozoa",
abstract = "Fifty ejaculates from donors serving on an AID panel were divided into two 0.5 aliquots and evaluated for initial motility and kinetics. The first aliquot was then diluted with Tyrode solution and incubated in a Tyrode atmosphere at 37°C. Motility and kinetics were evaluated at 1, 3, 6, and 9 hr. The second aliquot was frozen and thawed 24 hr later. The aliquot was then diluted with Tyrode solution, incubated, and evaluated as before. The results indicate a significant reduction in motility longevity of the thawed aliquots. A significant reduction in kinetics of the thawed aliquots was also discovered. These findings suggest that decreased motility longevity of thawed semen may offer an explanation for reduced pregnancy rates using cryopreserved semen.",
keywords = "Frozen Spermatozoa, Sperm, Sperm motility",
author = "Keel, {Brooks Allen} and Black, {J. B.}",
year = "1980",
month = "1",
day = "1",
doi = "10.3109/01485018008986965",
language = "English (US)",
volume = "4",
pages = "213--215",
journal = "Systems Biology in Reproductive Medicine",
issn = "1939-6368",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Reduced motility longevity in thawed human spermatozoa

AU - Keel, Brooks Allen

AU - Black, J. B.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - Fifty ejaculates from donors serving on an AID panel were divided into two 0.5 aliquots and evaluated for initial motility and kinetics. The first aliquot was then diluted with Tyrode solution and incubated in a Tyrode atmosphere at 37°C. Motility and kinetics were evaluated at 1, 3, 6, and 9 hr. The second aliquot was frozen and thawed 24 hr later. The aliquot was then diluted with Tyrode solution, incubated, and evaluated as before. The results indicate a significant reduction in motility longevity of the thawed aliquots. A significant reduction in kinetics of the thawed aliquots was also discovered. These findings suggest that decreased motility longevity of thawed semen may offer an explanation for reduced pregnancy rates using cryopreserved semen.

AB - Fifty ejaculates from donors serving on an AID panel were divided into two 0.5 aliquots and evaluated for initial motility and kinetics. The first aliquot was then diluted with Tyrode solution and incubated in a Tyrode atmosphere at 37°C. Motility and kinetics were evaluated at 1, 3, 6, and 9 hr. The second aliquot was frozen and thawed 24 hr later. The aliquot was then diluted with Tyrode solution, incubated, and evaluated as before. The results indicate a significant reduction in motility longevity of the thawed aliquots. A significant reduction in kinetics of the thawed aliquots was also discovered. These findings suggest that decreased motility longevity of thawed semen may offer an explanation for reduced pregnancy rates using cryopreserved semen.

KW - Frozen Spermatozoa

KW - Sperm

KW - Sperm motility

UR - http://www.scopus.com/inward/record.url?scp=84907038056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84907038056&partnerID=8YFLogxK

U2 - 10.3109/01485018008986965

DO - 10.3109/01485018008986965

M3 - Article

C2 - 7416844

AN - SCOPUS:84907038056

VL - 4

SP - 213

EP - 215

JO - Systems Biology in Reproductive Medicine

JF - Systems Biology in Reproductive Medicine

SN - 1939-6368

IS - 3

ER -