Reduced response of prostate cancer cells to TRAIL is modulated by NFkappaB-mediated inhibition of caspases and Bid activation.

Manal A. Eid, Ronald W Lewis, Asim B. Abdel-Mageed, M. Vijay Kumar

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We describe the effects of tumor necrosis factor alpha-related apoptosis inducing ligand (TRAIL) on the induction of apoptosis in two related prostate cancer cell lines, PC3AR and PC3Neo. TRAIL is a potent drug, which induces apoptosis preferentially in cancer cells. Treatment of prostate cancer cells, reduced survival by approximately 41% in PC3AR, but only approximately 18% PC3Neo were killed. Western analysis demonstrated that increased apoptotic response of PC3AR cells may be due to differential response of death receptors DR4, DR5 and decoy receptors DcR1 and DcR2. Caspases-8, -9, -3 and Bid were highly activated in PC3AR cells compared to PC3Neo. Furthermore, lower apoptotic response of PC3Neo was probably due to higher expression of NFkappaB. Blocking the function of NFkappaB by adenoviral infection of mutated IkappaB, increased apoptotic response confirming the influence of NFkappaB. Thus, we have demonstrated the role of NFkappaB in the differential response of prostate cancer cells to TRAIL.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalInternational Journal of Oncology
Volume21
Issue number1
StatePublished - Jan 1 2002

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Caspases
Prostatic Neoplasms
Apoptosis
TNF-Related Apoptosis-Inducing Ligand Receptors
Death Domain Receptors
Caspase 9
Caspase 8
Cell Survival
Tumor Necrosis Factor-alpha
Ligands
Cell Line
Infection
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Reduced response of prostate cancer cells to TRAIL is modulated by NFkappaB-mediated inhibition of caspases and Bid activation. / Eid, Manal A.; Lewis, Ronald W; Abdel-Mageed, Asim B.; Kumar, M. Vijay.

In: International Journal of Oncology, Vol. 21, No. 1, 01.01.2002, p. 111-117.

Research output: Contribution to journalArticle

Eid, Manal A. ; Lewis, Ronald W ; Abdel-Mageed, Asim B. ; Kumar, M. Vijay. / Reduced response of prostate cancer cells to TRAIL is modulated by NFkappaB-mediated inhibition of caspases and Bid activation. In: International Journal of Oncology. 2002 ; Vol. 21, No. 1. pp. 111-117.
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