Refined structure of rat Clara cell 17 kDa protein at 3·0 Å resolution

Timothy C. Umland, S. Swaminathan, William Furey, Gurmukh Singh, James Pletcher, Martin Sax

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The rat Clara cell 17 kDa protein (previously referred to as the rat Clara cell 10 kDa protein) has been reported to inhibit phospholipase A2 and papain, and to also bind progesterone. It has been isolated from rat lung lavage fluid and crystallized in the space group P6522. The structure has been determined to 3·0 Å resolution using the molecular replacement method. Uteroglobin, whose amino acid sequence is 55·7% identical, was used as the search model. The structure was then refined using restrained least-squares and simulated annealing methods. The R-factor is 22·5%. The protein is a covalently bound dimer. Two disulfide bonds join the monomers together in an antiparallel manner such that the dimer encloses a large internal hydrophobic cavity. The hydrophobic cavity is large enough to serve as the progesterone binding site, but access to the cavity is limited. Each monomer is composed of four α-helices. The main-chain structure of the Clara cell protein closely resembles that of uteroglobin, but the nature of many of the exposed side-chains differ. This is true, particularly in a hypervariable region between residues 23 and 36, and in the H1H4 pocket.

Original languageEnglish (US)
Pages (from-to)441-448
Number of pages8
JournalJournal of Molecular Biology
Volume224
Issue number2
DOIs
StatePublished - Mar 20 1992
Externally publishedYes

Fingerprint

Uteroglobin
Progesterone
R388
Proteins
Papain
Phospholipases A2
Bronchoalveolar Lavage Fluid
Least-Squares Analysis
Disulfides
Amino Acid Sequence
Binding Sites

Keywords

  • Clara cell 10 kDa protein
  • Clara cell 17 kDa protein
  • progesterone
  • uteroglobin

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Refined structure of rat Clara cell 17 kDa protein at 3·0 Å resolution. / Umland, Timothy C.; Swaminathan, S.; Furey, William; Singh, Gurmukh; Pletcher, James; Sax, Martin.

In: Journal of Molecular Biology, Vol. 224, No. 2, 20.03.1992, p. 441-448.

Research output: Contribution to journalArticle

Umland, Timothy C. ; Swaminathan, S. ; Furey, William ; Singh, Gurmukh ; Pletcher, James ; Sax, Martin. / Refined structure of rat Clara cell 17 kDa protein at 3·0 Å resolution. In: Journal of Molecular Biology. 1992 ; Vol. 224, No. 2. pp. 441-448.
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