Regional assignment of EST sequences on human chromosome 13

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have used a panel of somatic cell hybrids carrying structural rearrangements of human chromosome 13 to regionally localise 10 expressed sequence tags (ESTs) from this chromosome. Three of these genes were not present in the somatic cell hybrid PGME, which contains chromosome 13 as its major human component. Using a panel of somatic cell hybrids, two of these genes were localised to chromosome 8 and one to chromosome 16. Using the PCR primers as published, it was impossible to map one EST; consequently, a YAC containing this gene was isolated, and fluorescence in situ hybridisation was used to map the gene to 13q14. The distribution of the remaining six chromosome 13-specific ESTs was nonrandom; one mapped to 13q14 and the remaining five to 13q12, with three mapping within the proximal portion and two mapping within the distal portion of this region.

Original languageEnglish (US)
Pages (from-to)72-77
Number of pages6
JournalCytogenetic and Genome Research
Volume72
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Chromosomes, Human, Pair 13
Expressed Sequence Tags
Human Chromosomes
Hybrid Cells
propylene glycol methyl ether
Genes
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 8
Fluorescence In Situ Hybridization
Chromosomes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Regional assignment of EST sequences on human chromosome 13. / Hawthorn, Lesleyann; Cowell, John Kenneth.

In: Cytogenetic and Genome Research, Vol. 72, No. 1, 01.01.1996, p. 72-77.

Research output: Contribution to journalArticle

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