Regional cell movement and tissue patterning in the zebrafish embryo revealed by fate mapping with caged fluorescein

David J Kozlowski, Tohru Murakami, Robert K. Ho, Eric S. Weinberg

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Determination of fate maps and cell lineage tracing have previously been carried out in the zebrafish embryo by following the progeny of individual cells injected with fluorescent dyes. We review the information obtained from these experiments and then present an approach to fate mapping and cell movement tracing, utilizing the activation of caged fluorescein-dextran. This method has several advantages over single-cell injections in that it is rapid, allows cells at all depths in the embryo to be marked, can be used to follow cells starting at any time during development, and allows an appreciation of the movements of cells located in a coherent group at the time of uncaging. We demonstrate that the approach is effective in providing additional and complementary information on prospective mesoderm and brain tissues studied previously. We also present, for the first time, a fate map of placodal tissues including the otic vesicle, lateral line, cranial ganglia, lens, and olfactory epithelium. The prospective placodal cells are oriented at the 50% epiboly stage on the ventral side of the embryo with anterior structures close to the animal pole, and posterior structures nearer to the germ ring.

Original languageEnglish (US)
Pages (from-to)551-562
Number of pages12
JournalBiochemistry and Cell Biology
Volume75
Issue number5
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Fingerprint

Zebrafish
Fluorescein
Cell Movement
Embryonic Structures
Cells
Tissue
Fluorescent Dyes
Poles
Lenses
Brain
Animals
Chemical activation
Olfactory Mucosa
Cell Lineage
Mesoderm
Ganglia
Experiments
Ear
Injections
fluorescein-dextran

Keywords

  • Forebrain
  • Hindbrain
  • Inner ear
  • Midbrain
  • Otic vesicle
  • Placodes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Regional cell movement and tissue patterning in the zebrafish embryo revealed by fate mapping with caged fluorescein. / Kozlowski, David J; Murakami, Tohru; Ho, Robert K.; Weinberg, Eric S.

In: Biochemistry and Cell Biology, Vol. 75, No. 5, 01.01.1997, p. 551-562.

Research output: Contribution to journalArticle

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