Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain

Clare M Bergson, Ladislav Mrzljak, John F. Smiley, Mariamma Pappy, Robert Levenson, Patricia S. Goldman-Rakic

Research output: Contribution to journalArticle

385 Citations (Scopus)

Abstract

The pathways governing signal transduction in the mesocortical and nigrostriatal dopamine systems of the brain are of central importance in a variety of drug actions and neurological diseases. We have analyzed the regional, cellular, and subcellular distribution of the closely related D1 and D5 subtypes of dopamine receptors in the cerebral cortex and selected subcortical structures of rhesus monkey using subtype specific antibodies. The distribution of D1 and D5 receptors was highly differentiated in subcortical structures. In the neostriatum, both D1 and to a lesser extent Ds antibodies labeled medium spiny neurons, while only D5 antibodies labeled the large aspiny neurons typical of cholinergic interneurons. In the caudate nucleus, D1 labeling was concentrated in the spines and shafts of projection neurons, whereas D5 antibodies predominantly labeled the shafts, and less commonly, the spines of these cells. The D1 receptor was abundantly expressed in the neuropil of the substantia nigra pars reticulata while the D5 antibodies labeled only a few scattered cell bodies in this structure. Conversely, D5 antibodies labeled cholinergic neurons in the basal forebrain more intensely than D1 antibodies. Within the cerebral cortex and hippocampus, D1 and D5 antibody labeling was prominent in pyramidal cells. Double-label experiments revealed that the two receptors were frequently coexpressed in neurons of both structures. Ultrastructurally, D1 receptors were especially prominent in dendritic spines whereas dendritic shafts were more prominently labeled by the D5 receptor. The anatomical segregation of the D1 and D5 receptors at the subcellular level in cerebral cortex and at the cellular level in subcortical areas suggest that these closely related receptors may be preferentially associated with different circuit elements and may play distinct regulatory roles in synaptic transmission.

Original languageEnglish (US)
Pages (from-to)7821-7836
Number of pages16
JournalJournal of Neuroscience
Volume15
Issue number12
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

Dopamine D5 Receptors
Dopamine D1 Receptors
Primates
Antibodies
Brain
Cerebral Cortex
Cholinergic Neurons
Neurons
Spine
Neostriatum
Dendritic Spines
Neuropil
Pyramidal Cells
Caudate Nucleus
Interneurons
Macaca mulatta
Synaptic Transmission
Signal Transduction
Hippocampus
Dopamine

Keywords

  • Western blotting
  • antibody
  • electron microscopy
  • immunofluorescence
  • prefrontal cortex
  • working memory

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bergson, C. M., Mrzljak, L., Smiley, J. F., Pappy, M., Levenson, R., & Goldman-Rakic, P. S. (1995). Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain. Journal of Neuroscience, 15(12), 7821-7836.

Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain. / Bergson, Clare M; Mrzljak, Ladislav; Smiley, John F.; Pappy, Mariamma; Levenson, Robert; Goldman-Rakic, Patricia S.

In: Journal of Neuroscience, Vol. 15, No. 12, 01.12.1995, p. 7821-7836.

Research output: Contribution to journalArticle

Bergson, CM, Mrzljak, L, Smiley, JF, Pappy, M, Levenson, R & Goldman-Rakic, PS 1995, 'Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain', Journal of Neuroscience, vol. 15, no. 12, pp. 7821-7836.
Bergson, Clare M ; Mrzljak, Ladislav ; Smiley, John F. ; Pappy, Mariamma ; Levenson, Robert ; Goldman-Rakic, Patricia S. / Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain. In: Journal of Neuroscience. 1995 ; Vol. 15, No. 12. pp. 7821-7836.
@article{81cb403edc474a99a485d03be7cce4bd,
title = "Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain",
abstract = "The pathways governing signal transduction in the mesocortical and nigrostriatal dopamine systems of the brain are of central importance in a variety of drug actions and neurological diseases. We have analyzed the regional, cellular, and subcellular distribution of the closely related D1 and D5 subtypes of dopamine receptors in the cerebral cortex and selected subcortical structures of rhesus monkey using subtype specific antibodies. The distribution of D1 and D5 receptors was highly differentiated in subcortical structures. In the neostriatum, both D1 and to a lesser extent Ds antibodies labeled medium spiny neurons, while only D5 antibodies labeled the large aspiny neurons typical of cholinergic interneurons. In the caudate nucleus, D1 labeling was concentrated in the spines and shafts of projection neurons, whereas D5 antibodies predominantly labeled the shafts, and less commonly, the spines of these cells. The D1 receptor was abundantly expressed in the neuropil of the substantia nigra pars reticulata while the D5 antibodies labeled only a few scattered cell bodies in this structure. Conversely, D5 antibodies labeled cholinergic neurons in the basal forebrain more intensely than D1 antibodies. Within the cerebral cortex and hippocampus, D1 and D5 antibody labeling was prominent in pyramidal cells. Double-label experiments revealed that the two receptors were frequently coexpressed in neurons of both structures. Ultrastructurally, D1 receptors were especially prominent in dendritic spines whereas dendritic shafts were more prominently labeled by the D5 receptor. The anatomical segregation of the D1 and D5 receptors at the subcellular level in cerebral cortex and at the cellular level in subcortical areas suggest that these closely related receptors may be preferentially associated with different circuit elements and may play distinct regulatory roles in synaptic transmission.",
keywords = "Western blotting, antibody, electron microscopy, immunofluorescence, prefrontal cortex, working memory",
author = "Bergson, {Clare M} and Ladislav Mrzljak and Smiley, {John F.} and Mariamma Pappy and Robert Levenson and Goldman-Rakic, {Patricia S.}",
year = "1995",
month = "12",
day = "1",
language = "English (US)",
volume = "15",
pages = "7821--7836",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "12",

}

TY - JOUR

T1 - Regional, cellular, and subcellular variations in the distribution of D1 and D5 dopamine receptors in primate brain

AU - Bergson, Clare M

AU - Mrzljak, Ladislav

AU - Smiley, John F.

AU - Pappy, Mariamma

AU - Levenson, Robert

AU - Goldman-Rakic, Patricia S.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - The pathways governing signal transduction in the mesocortical and nigrostriatal dopamine systems of the brain are of central importance in a variety of drug actions and neurological diseases. We have analyzed the regional, cellular, and subcellular distribution of the closely related D1 and D5 subtypes of dopamine receptors in the cerebral cortex and selected subcortical structures of rhesus monkey using subtype specific antibodies. The distribution of D1 and D5 receptors was highly differentiated in subcortical structures. In the neostriatum, both D1 and to a lesser extent Ds antibodies labeled medium spiny neurons, while only D5 antibodies labeled the large aspiny neurons typical of cholinergic interneurons. In the caudate nucleus, D1 labeling was concentrated in the spines and shafts of projection neurons, whereas D5 antibodies predominantly labeled the shafts, and less commonly, the spines of these cells. The D1 receptor was abundantly expressed in the neuropil of the substantia nigra pars reticulata while the D5 antibodies labeled only a few scattered cell bodies in this structure. Conversely, D5 antibodies labeled cholinergic neurons in the basal forebrain more intensely than D1 antibodies. Within the cerebral cortex and hippocampus, D1 and D5 antibody labeling was prominent in pyramidal cells. Double-label experiments revealed that the two receptors were frequently coexpressed in neurons of both structures. Ultrastructurally, D1 receptors were especially prominent in dendritic spines whereas dendritic shafts were more prominently labeled by the D5 receptor. The anatomical segregation of the D1 and D5 receptors at the subcellular level in cerebral cortex and at the cellular level in subcortical areas suggest that these closely related receptors may be preferentially associated with different circuit elements and may play distinct regulatory roles in synaptic transmission.

AB - The pathways governing signal transduction in the mesocortical and nigrostriatal dopamine systems of the brain are of central importance in a variety of drug actions and neurological diseases. We have analyzed the regional, cellular, and subcellular distribution of the closely related D1 and D5 subtypes of dopamine receptors in the cerebral cortex and selected subcortical structures of rhesus monkey using subtype specific antibodies. The distribution of D1 and D5 receptors was highly differentiated in subcortical structures. In the neostriatum, both D1 and to a lesser extent Ds antibodies labeled medium spiny neurons, while only D5 antibodies labeled the large aspiny neurons typical of cholinergic interneurons. In the caudate nucleus, D1 labeling was concentrated in the spines and shafts of projection neurons, whereas D5 antibodies predominantly labeled the shafts, and less commonly, the spines of these cells. The D1 receptor was abundantly expressed in the neuropil of the substantia nigra pars reticulata while the D5 antibodies labeled only a few scattered cell bodies in this structure. Conversely, D5 antibodies labeled cholinergic neurons in the basal forebrain more intensely than D1 antibodies. Within the cerebral cortex and hippocampus, D1 and D5 antibody labeling was prominent in pyramidal cells. Double-label experiments revealed that the two receptors were frequently coexpressed in neurons of both structures. Ultrastructurally, D1 receptors were especially prominent in dendritic spines whereas dendritic shafts were more prominently labeled by the D5 receptor. The anatomical segregation of the D1 and D5 receptors at the subcellular level in cerebral cortex and at the cellular level in subcortical areas suggest that these closely related receptors may be preferentially associated with different circuit elements and may play distinct regulatory roles in synaptic transmission.

KW - Western blotting

KW - antibody

KW - electron microscopy

KW - immunofluorescence

KW - prefrontal cortex

KW - working memory

UR - http://www.scopus.com/inward/record.url?scp=0029560902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029560902&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 7821

EP - 7836

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 12

ER -