Regional distribution and cellular localization of γ-aminobutyric acid subtype 1 receptor mRNA in the rat brain

Xinyun Lu, M. Behnam Ghasemzadeh, Peter W. Kalivas

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The distribution of γ-aminobutyric acid (GABA) receptor subtype B1 (GABA(B1)) mRNA-containing cells in the brain of adult rats was determined with in situ hybridization histochemistry. The vast majority of neurons expressed GABA(B1) receptor mRNA. However, there were nuclei of relative high density, and, in some nuclei, the majority of neurons did not express detectable levels of GABA(B1) receptor transcripts. Areas where the majority of neurons expressed a high density of mRNA included the medial habenula; the septohippocampal, periventricular, suprachiasmatic, and supraoptic nuclei; Purkinje cells in the cerebellum; and pyramidal and granule cells of the hippocampus and dentate gyrus, respectively. Also, brainstem nuclei containing monoaminergic neurons and neurons in the thalamic motor nuclei contained relatively high levels of expression, mRNA was low or absent in neuronal populations in regions with well-developed cytoarchitecture, such as the stratum radiatum and stratum oriens of the hippocampus, the molecular and granular layers of the cerebellum, and the molecular layer of the cortex. Low expression was observed also in many extrapyramidal nuclei, such as the globus and ventral pallidum and the substantia nigra, pars reticulata. Expression also was low in the reticular thalamic nucleus and zona incerta. Neurons lacking detectable GABA(B1) receptor mRNA were generally in nuclei that contained largely GABAergic neurons.

Original languageEnglish (US)
Pages (from-to)166-182
Number of pages17
JournalJournal of Comparative Neurology
Volume407
Issue number2
DOIs
StatePublished - May 3 1999
Externally publishedYes

Fingerprint

Aminobutyrates
GABA Receptors
Neurons
Messenger RNA
Brain
Thalamic Nuclei
Cerebellum
Hippocampus
Habenula
GABAergic Neurons
Supraoptic Nucleus
Suprachiasmatic Nucleus
Specific Gravity
Pyramidal Cells
Purkinje Cells
Dentate Gyrus
gamma-Aminobutyric Acid
Brain Stem
In Situ Hybridization
Population

Keywords

  • Cerebellum
  • In situ hybridization
  • Locus coeruleus
  • Raphe
  • Thalamus
  • Ventral mesencephalon

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regional distribution and cellular localization of γ-aminobutyric acid subtype 1 receptor mRNA in the rat brain. / Lu, Xinyun; Ghasemzadeh, M. Behnam; Kalivas, Peter W.

In: Journal of Comparative Neurology, Vol. 407, No. 2, 03.05.1999, p. 166-182.

Research output: Contribution to journalArticle

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