Regulation of α2B-Adrenergic Receptor Cell Surface Transport by GGA1 and GGA2

Maoxiang Zhang, Wei Huang, Jie Gao, Alvin V Terry, Guangyu Wu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The molecular mechanisms that control the targeting of newly synthesized G protein-coupled receptors (GPCRs) to the functional destinations remain poorly elucidated. Here, we have determined the role of Golgi-localized, γ-adaptin ear domain homology, ADP ribosylation factor-binding proteins 1 and 2 (GGA1 and GGA2) in the cell surface transport of α2B-adrenergic receptor (α2B-AR), a prototypic GPCR, and studied the underlying mechanisms. We demonstrated that knockdown of GGA1 and GGA2 by shRNA and siRNA significantly reduced the cell surface expression of inducibly expressed α2B-AR and arrested the receptor in the perinuclear region. Knockdown of each GGA markedly inhibited the dendritic expression of α2B-AR in primary cortical neurons. Consistently, depleting GGA1 and GGA2 attenuated receptor-mediated signal transduction measured as ERK1/2 activation and cAMP inhibition. Although full length α2B-AR associated with GGA2 but not GGA1, its third intracellular loop was found to directly interact with both GGA1 and GGA2. More interestingly, further mapping of interaction domains showed that the GGA1 hinge region and the GGA2 GAE domain bound to multiple subdomains of the loop. These studies have identified an important function and revealed novel mechanisms of the GGA family proteins in the forward trafficking of a cell surface GPCR.

Original languageEnglish (US)
Article number37921
JournalScientific Reports
Volume6
DOIs
StatePublished - Nov 30 2016

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Adrenergic Receptors
G-Protein-Coupled Receptors
Small Interfering RNA
ADP-Ribosylation Factors
Ear
Signal Transduction
Carrier Proteins
Membrane Proteins
Neurons
Proteins

ASJC Scopus subject areas

  • General

Cite this

Regulation of α2B-Adrenergic Receptor Cell Surface Transport by GGA1 and GGA2. / Zhang, Maoxiang; Huang, Wei; Gao, Jie; Terry, Alvin V; Wu, Guangyu.

In: Scientific Reports, Vol. 6, 37921, 30.11.2016.

Research output: Contribution to journalArticle

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