Regulation of adiponectin secretion by adipocytes in the polycystic ovary syndrome: Role of tumor necrosis factor-α

Gregorio Chazenbalk, Bradley S. Trivax, Bulent O. Yildiz, Cristina Bertolotto, Ruchi Mathur, Saleh Heneidi, Ricardo Azziz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Context: Adipose tissue dysfunction associated with low-grade chronic inflammation and dysregulation of adipokine secretion might significantly contribute to the pathogenesis of polycystic ovary syndrome (PCOS). Objective: The objective of the study was to determine whether the effect of TNF-α, IL-6, monocyte chemoattractant protein-1, or coculture of adipocytes and adipose tissue macrophages (ATMs), on the secretion of adiponectin by adipocytes, differs in PCOS compared with controls. Design and Participants: Primary cultures of sc adipocytes and coculture of adipocytes and ATMs from overweight and obese patients with PCOS and healthy control women were used. Main Outcome Measures: Adiponectin secretion by adipocytes was measured. Results: The baseline secretion of adiponectin by isolated adipocytes did not differ between PCOS and control samples. The net change in adiponectin secretion in response to IL-6, monocyte chemoattractant protein-1, and TNF-α differed between PCOS (decreasing) and control (increasing) adipocytes, although the difference reached significance only for TNF-α (P < 0.04). Coculture of isolated adipocytes and ATMs resulted in a decrease in adiponectin secretion by PCOS (P < 0.05) but not control adipocytes, and the difference between the net change in adiponectin secretion in PCOS vs. control samples was significant (P < 0.03). Conclusions: Our results suggest that adiponectin secretion by adipocytes in response to cytokines/ chemokines and most notably in response to coculturing with ATMs differs between PCOS and control women, favoring greater suppression of adiponectin in PCOS. The mechanisms underlying these defects and the role of concurrent obesity remain to be determined.

Original languageEnglish (US)
Pages (from-to)935-942
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Volume95
Issue number2
DOIs
StatePublished - Feb 2010

Fingerprint

Polycystic Ovary Syndrome
Adiponectin
Adipocytes
Tumor Necrosis Factor-alpha
Macrophages
Tissue
Adipose Tissue
Coculture Techniques
Chemokine CCL2
Interleukin-6
Adipokines
Chemokines
Cytokines
Defects
Obesity
Outcome Assessment (Health Care)
Inflammation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Regulation of adiponectin secretion by adipocytes in the polycystic ovary syndrome : Role of tumor necrosis factor-α. / Chazenbalk, Gregorio; Trivax, Bradley S.; Yildiz, Bulent O.; Bertolotto, Cristina; Mathur, Ruchi; Heneidi, Saleh; Azziz, Ricardo.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 95, No. 2, 02.2010, p. 935-942.

Research output: Contribution to journalArticle

Chazenbalk, Gregorio ; Trivax, Bradley S. ; Yildiz, Bulent O. ; Bertolotto, Cristina ; Mathur, Ruchi ; Heneidi, Saleh ; Azziz, Ricardo. / Regulation of adiponectin secretion by adipocytes in the polycystic ovary syndrome : Role of tumor necrosis factor-α. In: Journal of Clinical Endocrinology and Metabolism. 2010 ; Vol. 95, No. 2. pp. 935-942.
@article{a5fa8042c75247d397298d273d9b881a,
title = "Regulation of adiponectin secretion by adipocytes in the polycystic ovary syndrome: Role of tumor necrosis factor-α",
abstract = "Context: Adipose tissue dysfunction associated with low-grade chronic inflammation and dysregulation of adipokine secretion might significantly contribute to the pathogenesis of polycystic ovary syndrome (PCOS). Objective: The objective of the study was to determine whether the effect of TNF-α, IL-6, monocyte chemoattractant protein-1, or coculture of adipocytes and adipose tissue macrophages (ATMs), on the secretion of adiponectin by adipocytes, differs in PCOS compared with controls. Design and Participants: Primary cultures of sc adipocytes and coculture of adipocytes and ATMs from overweight and obese patients with PCOS and healthy control women were used. Main Outcome Measures: Adiponectin secretion by adipocytes was measured. Results: The baseline secretion of adiponectin by isolated adipocytes did not differ between PCOS and control samples. The net change in adiponectin secretion in response to IL-6, monocyte chemoattractant protein-1, and TNF-α differed between PCOS (decreasing) and control (increasing) adipocytes, although the difference reached significance only for TNF-α (P < 0.04). Coculture of isolated adipocytes and ATMs resulted in a decrease in adiponectin secretion by PCOS (P < 0.05) but not control adipocytes, and the difference between the net change in adiponectin secretion in PCOS vs. control samples was significant (P < 0.03). Conclusions: Our results suggest that adiponectin secretion by adipocytes in response to cytokines/ chemokines and most notably in response to coculturing with ATMs differs between PCOS and control women, favoring greater suppression of adiponectin in PCOS. The mechanisms underlying these defects and the role of concurrent obesity remain to be determined.",
author = "Gregorio Chazenbalk and Trivax, {Bradley S.} and Yildiz, {Bulent O.} and Cristina Bertolotto and Ruchi Mathur and Saleh Heneidi and Ricardo Azziz",
year = "2010",
month = "2",
doi = "10.1210/jc.2009-1158",
language = "English (US)",
volume = "95",
pages = "935--942",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "2",

}

TY - JOUR

T1 - Regulation of adiponectin secretion by adipocytes in the polycystic ovary syndrome

T2 - Role of tumor necrosis factor-α

AU - Chazenbalk, Gregorio

AU - Trivax, Bradley S.

AU - Yildiz, Bulent O.

AU - Bertolotto, Cristina

AU - Mathur, Ruchi

AU - Heneidi, Saleh

AU - Azziz, Ricardo

PY - 2010/2

Y1 - 2010/2

N2 - Context: Adipose tissue dysfunction associated with low-grade chronic inflammation and dysregulation of adipokine secretion might significantly contribute to the pathogenesis of polycystic ovary syndrome (PCOS). Objective: The objective of the study was to determine whether the effect of TNF-α, IL-6, monocyte chemoattractant protein-1, or coculture of adipocytes and adipose tissue macrophages (ATMs), on the secretion of adiponectin by adipocytes, differs in PCOS compared with controls. Design and Participants: Primary cultures of sc adipocytes and coculture of adipocytes and ATMs from overweight and obese patients with PCOS and healthy control women were used. Main Outcome Measures: Adiponectin secretion by adipocytes was measured. Results: The baseline secretion of adiponectin by isolated adipocytes did not differ between PCOS and control samples. The net change in adiponectin secretion in response to IL-6, monocyte chemoattractant protein-1, and TNF-α differed between PCOS (decreasing) and control (increasing) adipocytes, although the difference reached significance only for TNF-α (P < 0.04). Coculture of isolated adipocytes and ATMs resulted in a decrease in adiponectin secretion by PCOS (P < 0.05) but not control adipocytes, and the difference between the net change in adiponectin secretion in PCOS vs. control samples was significant (P < 0.03). Conclusions: Our results suggest that adiponectin secretion by adipocytes in response to cytokines/ chemokines and most notably in response to coculturing with ATMs differs between PCOS and control women, favoring greater suppression of adiponectin in PCOS. The mechanisms underlying these defects and the role of concurrent obesity remain to be determined.

AB - Context: Adipose tissue dysfunction associated with low-grade chronic inflammation and dysregulation of adipokine secretion might significantly contribute to the pathogenesis of polycystic ovary syndrome (PCOS). Objective: The objective of the study was to determine whether the effect of TNF-α, IL-6, monocyte chemoattractant protein-1, or coculture of adipocytes and adipose tissue macrophages (ATMs), on the secretion of adiponectin by adipocytes, differs in PCOS compared with controls. Design and Participants: Primary cultures of sc adipocytes and coculture of adipocytes and ATMs from overweight and obese patients with PCOS and healthy control women were used. Main Outcome Measures: Adiponectin secretion by adipocytes was measured. Results: The baseline secretion of adiponectin by isolated adipocytes did not differ between PCOS and control samples. The net change in adiponectin secretion in response to IL-6, monocyte chemoattractant protein-1, and TNF-α differed between PCOS (decreasing) and control (increasing) adipocytes, although the difference reached significance only for TNF-α (P < 0.04). Coculture of isolated adipocytes and ATMs resulted in a decrease in adiponectin secretion by PCOS (P < 0.05) but not control adipocytes, and the difference between the net change in adiponectin secretion in PCOS vs. control samples was significant (P < 0.03). Conclusions: Our results suggest that adiponectin secretion by adipocytes in response to cytokines/ chemokines and most notably in response to coculturing with ATMs differs between PCOS and control women, favoring greater suppression of adiponectin in PCOS. The mechanisms underlying these defects and the role of concurrent obesity remain to be determined.

UR - http://www.scopus.com/inward/record.url?scp=76149112554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76149112554&partnerID=8YFLogxK

U2 - 10.1210/jc.2009-1158

DO - 10.1210/jc.2009-1158

M3 - Article

C2 - 20089616

AN - SCOPUS:76149112554

VL - 95

SP - 935

EP - 942

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 2

ER -