Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells

Haiying Liang, Virginia J. Venema, Xiaodan Wang, Hong Ju, Richard C Venema, Mario B Marrero

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Ligand binding to the angiotensin II (Ang II) AT1 receptor on vascular smooth muscle cells (VSMCs) activates the Janus-activated kinase (JAK)/signal transducers and activators of transcription (STAT) pathway. We have shown previously that the JAK2 tyrosine kinase and the Src family p59 Fyn tyrosine kinase are required for Ang II-induced STAT1 tyrosine phosphorylation in VSMCs. The mitogen-activated protein kinase phosphatase, MKP-1, is required for STAT1 tyrosine dephosphorylation. In the present study, using specific enzyme inhibitors and antisense oligonucleotides, we show that Ang II- induced tyrosine phosphorylation and nuclear translocation of STAT3 in VSMCs is mediated by p60 c-Src, whereas tyrosine dephosphorylation is mediated by calcineurin. Calcineurin is activated in response to Ang II stimulation of VSMCs and is translocated to the nucleus. In addition, we show that Ang II- induced serine phosphorylation of STAT3 in VSMCs is mediated by mitogen- activated protein kinase and that dephosphorylation is mediated by protein phosphatase 2A (PP2A). PP2A translocates to the nucleus in response to Ang II stimulation of VSMCs and forms a complex with STAT3 in an Ang II-dependent manner.

Original languageEnglish (US)
Pages (from-to)19846-19851
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number28
DOIs
StatePublished - Jul 9 1999

Fingerprint

Phosphorylation
Vascular Smooth Muscle
Angiotensin II
Smooth Muscle Myocytes
Muscle
Cells
Tyrosine
Protein Phosphatase 2
Calcineurin
Proto-Oncogene Proteins c-fyn
Mitogen-Activated Protein Kinase Phosphatases
Janus Kinases
Angiotensin Type 1 Receptor
Angiotensin Receptors
src-Family Kinases
Antisense Oligonucleotides
Enzyme Inhibitors
Mitogen-Activated Protein Kinases
Transducers
Serine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Liang, H., Venema, V. J., Wang, X., Ju, H., Venema, R. C., & Marrero, M. B. (1999). Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells. Journal of Biological Chemistry, 274(28), 19846-19851. https://doi.org/10.1074/jbc.274.28.19846

Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells. / Liang, Haiying; Venema, Virginia J.; Wang, Xiaodan; Ju, Hong; Venema, Richard C; Marrero, Mario B.

In: Journal of Biological Chemistry, Vol. 274, No. 28, 09.07.1999, p. 19846-19851.

Research output: Contribution to journalArticle

Liang, H, Venema, VJ, Wang, X, Ju, H, Venema, RC & Marrero, MB 1999, 'Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells', Journal of Biological Chemistry, vol. 274, no. 28, pp. 19846-19851. https://doi.org/10.1074/jbc.274.28.19846
Liang, Haiying ; Venema, Virginia J. ; Wang, Xiaodan ; Ju, Hong ; Venema, Richard C ; Marrero, Mario B. / Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 28. pp. 19846-19851.
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