Regulation of E2F1-induced apoptosis by poly(ADP-ribosyl)ation

A. Kumari, T. Iwasaki, S. Pyndiah, E. K. Cassimere, C. D. Palani, Daitoku Sakamuro

Research output: Contribution to journalArticle

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Abstract

The transcription factor adenovirus E2 promoter-binding factor (E2F)-1 normally enhances cell-cycle progression, but it also induces apoptosis under certain conditions, including DNA damage and serum deprivation. Although DNA damage facilitates the phosphorylation and stabilization of E2F1 to trigger apoptosis, how serum starvation renders cells vulnerable to E2F1-induced apoptosis remains unclear. Because poly(ADP-ribose) polymerase 1 (PARP1), a nuclear enzyme essential for genomic stability and chromatin remodeling, interacts directly with E2F1, we investigated the effects of PARP1 on E2F1-mediated functions in the presence and absence of serum. PARP1 attenuation, which increased E2F1 transactivation, induced G 2/M cell-cycle arrest under normal growth conditions, but enhanced E2F1-induced apoptosis in serum-starved cells. Interestingly, basal PARP1 activity was sufficient to modify E2F1 by poly(ADP-ribosyl)ation, which stabilized the interaction between E2F1 and the BIN1 tumor suppressor in the nucleus. Accordingly, BIN1 acted as an RB1-independent E2F1 corepressor. Because E2F1 directly activates the BIN1 gene promoter, BIN1 curbed E2F1 activity through a negative-feedback mechanism. Conversely, when the BIN1-E2F1 interaction was abolished by PARP1 suppression, E2F1 continuously increased BIN1 levels. This is functionally germane, as PARP1-depletion-associated G 2/M arrest was reversed by the transfection of BIN1 siRNA. Moreover, PARP-inhibitor-associated anti-transformation activity was compromised by the coexpression of dominant-negative BIN1. Because serum starvation massively reduced the E2F1 poly(ADP-ribosyl)ation, we conclude that the release of BIN1 from hypo-poly(ADP-ribosyl)ated E2F1 is a mechanism by which serum starvation promotes E2F1-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)311-322
Number of pages12
JournalCell Death and Differentiation
Volume22
Issue number2
DOIs
StatePublished - Feb 1 2015

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Adenosine Diphosphate
Apoptosis
Starvation
Serum
DNA Damage
Co-Repressor Proteins
Chromatin Assembly and Disassembly
Genomic Instability
Cell Cycle Checkpoints
Adenoviridae
Small Interfering RNA
Transcriptional Activation
Transfection
Poly (ADP-Ribose) Polymerase-1
Cell Cycle
Transcription Factors
Phosphorylation
Enzymes
Growth
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Kumari, A., Iwasaki, T., Pyndiah, S., Cassimere, E. K., Palani, C. D., & Sakamuro, D. (2015). Regulation of E2F1-induced apoptosis by poly(ADP-ribosyl)ation. Cell Death and Differentiation, 22(2), 311-322. https://doi.org/10.1038/cdd.2014.146

Regulation of E2F1-induced apoptosis by poly(ADP-ribosyl)ation. / Kumari, A.; Iwasaki, T.; Pyndiah, S.; Cassimere, E. K.; Palani, C. D.; Sakamuro, Daitoku.

In: Cell Death and Differentiation, Vol. 22, No. 2, 01.02.2015, p. 311-322.

Research output: Contribution to journalArticle

Kumari, A, Iwasaki, T, Pyndiah, S, Cassimere, EK, Palani, CD & Sakamuro, D 2015, 'Regulation of E2F1-induced apoptosis by poly(ADP-ribosyl)ation', Cell Death and Differentiation, vol. 22, no. 2, pp. 311-322. https://doi.org/10.1038/cdd.2014.146
Kumari, A. ; Iwasaki, T. ; Pyndiah, S. ; Cassimere, E. K. ; Palani, C. D. ; Sakamuro, Daitoku. / Regulation of E2F1-induced apoptosis by poly(ADP-ribosyl)ation. In: Cell Death and Differentiation. 2015 ; Vol. 22, No. 2. pp. 311-322.
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